vo (umol/min) 20.8 Vu (µmol/min) 4.2 [Substrate] (M) 6 x 10 1x 10 29 5.8 2 x 103 45 9 6 x 103 67.6 13.6 1.8 x 104 87 16.2

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter17: Introduction To Iv Therapy
Section: Chapter Questions
Problem 3.6P
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Question

Consider the following data for an enzyme-catalyzed
hydrolysis reaction in the presence and absence of
inhibitor I:

Using a Michaelis-Menten plot, determine Km for the
uninhibited reaction and the inhibited reaction.

vo (umol/min)
20.8
Vu (µmol/min)
4.2
[Substrate] (M)
6 x 10
1x 10
29
5.8
2 x 103
45
9
6 x 103
67.6
13.6
1.8 x 104
87
16.2
Transcribed Image Text:vo (umol/min) 20.8 Vu (µmol/min) 4.2 [Substrate] (M) 6 x 10 1x 10 29 5.8 2 x 103 45 9 6 x 103 67.6 13.6 1.8 x 104 87 16.2
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