Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. ■ y-int = [Select] · slope = ▪ Vmax= Km = [ Select] [ Select] [Select] What is the type of inhibition involved? [Select] V ✓mM/min < mM

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Chapter1: Chemical Foundations
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Using linear regression analysis, determine the values of Vmax
and Km of the enzyme in the presence of an inhibitor.
y-int = [Select]
slope =
max
Kma
[Select]
[Select]
[Select]
V
What is the type of inhibition involved?
[Select]
V
mM/min
MM
Transcribed Image Text:Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. y-int = [Select] slope = max Kma [Select] [Select] [Select] V What is the type of inhibition involved? [Select] V mM/min MM
A biochemist wants to determine the effect of an inhibitor on a
certain enzyme. The data are shown below:
■
[Substrate]
(mm)
15
30
45
60
I
Vo (mm/min)
(without inhibitor)
Using linear regression analysis, determine the values of Vmax
and Km of the enzyme in the absence of an inhibitor.
y-int = [Select]
slope = [Select]
▪ Vmax= [Select]
Km
[ Select]
1.09
1.83
2.30
2.64
Vo (mm/min)
(with inhibitor)
V
0.63
1.13
1.53
1.85
mM/min
mM
Transcribed Image Text:A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: ■ [Substrate] (mm) 15 30 45 60 I Vo (mm/min) (without inhibitor) Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. y-int = [Select] slope = [Select] ▪ Vmax= [Select] Km [ Select] 1.09 1.83 2.30 2.64 Vo (mm/min) (with inhibitor) V 0.63 1.13 1.53 1.85 mM/min mM
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