Using absorbance at 234 nm, 280 nm, and 260 nm, you calculated the percent of proteins present in your DNA. The percent of proteins present when calculated using absorbance at 260 nm and 280 nm was much larger than the percent of proteins present calculated using absorbance at 260 nm and 234 nm. This indicated that: a. Peptides present in your DNA preparation contain a large number of amino acids, tyrosine, phenylalanine, and tryptophan. 5. Peptides present in your DNA preparation contain a small number of amino acids tyrosine, phenylalanine, and tryptophan. C. Peptides present in your DNA preparation do not contain amino acids tyrosine, phenylalanine, and tryptophan. d. Your DNA preparation is not contaminated with proteins. e. All of the above. f. None of the above.
Using absorbance at 234 nm, 280 nm, and 260 nm, you calculated the percent of proteins present in your DNA. The percent of proteins present when calculated using absorbance at 260 nm and 280 nm was much larger than the percent of proteins present calculated using absorbance at 260 nm and 234 nm. This indicated that: a. Peptides present in your DNA preparation contain a large number of amino acids, tyrosine, phenylalanine, and tryptophan. 5. Peptides present in your DNA preparation contain a small number of amino acids tyrosine, phenylalanine, and tryptophan. C. Peptides present in your DNA preparation do not contain amino acids tyrosine, phenylalanine, and tryptophan. d. Your DNA preparation is not contaminated with proteins. e. All of the above. f. None of the above.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Question
Propose an appropriate intermediate for the following reaction
Expert Solution
Step 1
Solutions-
UV-visible absorption spectroscopy for proteins and DNA
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY