Use the provided graph paper to construct a Beer's Law plot: absorbance versus concentration. Draw a best-fit line with a ruler or other straight edge. Submit this graph with your other report pages. Choose two locations on the best- fit line (not data points) and calculate the slope (show your work): slope = (Y2- Y1)/(X2 – X1) %3D TO 002)A41-55 0.002)/0002 Use the slope from #2 to calculate the concentration of your unknown: y = mx, where m = slope, and y is the absorbance provided by your instructor:

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Record the apparent color of the solution.
Velength and color of the light.
3.
4 Prepare standards: imagine that you will be preparing various dilutions of the stock solution for your solution, These
should include 1/4, 1/6, and 1/10 dilutions of the stock. For example, if your stock solution had a concentration of 900
mM, you would use the following solutions: 900 mM (stock), 225 mM (1/4), 150 mM (1/6), and 90 mM (1/10). Note the
concentration unit listed for your stock (it may be mM or µM). Calculate the appropriate values for your solution.
Move the sliding bar to the various concentrations you calculated, and record the absorbance.
5.
DATA TABLE
Standard
Standard conc.
Absorbance
CobAit L1)
solution CoCla Cenloride
Stock conc.
100mM
0-51
wavelength508nm
1/4 conc.
25 MM
0.13
color of light_GREEN
1/6 conc.
It:7MM
0.08
color of solution Ank
1/10 conc.
10 MM
0.05
Unknown #
9
Absorbance: |- 55
DATA ANALYSIS – WORK ALONE
1.
In a physical laboratory, you would have prepared your standards by dilution of the stock solution. Pick one of your
four dilutions, and calculate the volumes of water and stock solution you would combine to prepare 20.00 mL of that
standard (üse the dilution equation): m,N, = MeVz
(25 MM) = L10 omm) L 20.00ML)
V, = 80ML
2. Use the provided graph paper to construct a Beer's Law plot: absorbance versus concentration. Draw a best-fit line
with a ruler or other straight edge. Submit this graph with your other report pages. Choose two locations on the best-
fit line (not data points) and calculate the slope (show your work):
slope = (Y2- Y1)/(X2 – X1)
3. Use the slope from #2 to calculate the concentration of your unknown: y = mx, where m = slope, and y is the
absorbance provided by your instructor:
concentration
unknown #
Concen TRATION
GEA
CAPACITY
SHEET
Transcribed Image Text:Record the apparent color of the solution. Velength and color of the light. 3. 4 Prepare standards: imagine that you will be preparing various dilutions of the stock solution for your solution, These should include 1/4, 1/6, and 1/10 dilutions of the stock. For example, if your stock solution had a concentration of 900 mM, you would use the following solutions: 900 mM (stock), 225 mM (1/4), 150 mM (1/6), and 90 mM (1/10). Note the concentration unit listed for your stock (it may be mM or µM). Calculate the appropriate values for your solution. Move the sliding bar to the various concentrations you calculated, and record the absorbance. 5. DATA TABLE Standard Standard conc. Absorbance CobAit L1) solution CoCla Cenloride Stock conc. 100mM 0-51 wavelength508nm 1/4 conc. 25 MM 0.13 color of light_GREEN 1/6 conc. It:7MM 0.08 color of solution Ank 1/10 conc. 10 MM 0.05 Unknown # 9 Absorbance: |- 55 DATA ANALYSIS – WORK ALONE 1. In a physical laboratory, you would have prepared your standards by dilution of the stock solution. Pick one of your four dilutions, and calculate the volumes of water and stock solution you would combine to prepare 20.00 mL of that standard (üse the dilution equation): m,N, = MeVz (25 MM) = L10 omm) L 20.00ML) V, = 80ML 2. Use the provided graph paper to construct a Beer's Law plot: absorbance versus concentration. Draw a best-fit line with a ruler or other straight edge. Submit this graph with your other report pages. Choose two locations on the best- fit line (not data points) and calculate the slope (show your work): slope = (Y2- Y1)/(X2 – X1) 3. Use the slope from #2 to calculate the concentration of your unknown: y = mx, where m = slope, and y is the absorbance provided by your instructor: concentration unknown # Concen TRATION GEA CAPACITY SHEET
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