4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400 µL in each well. Calculate the volumes of each reagent that needs to be added and complete the table below (fill in the blue boxes). [BSA] µg/ BSA (µL) Bradford Total volume Absorbance Well Water (µL) Reagent (µL) OD 595nm (A595) (µL) A1 100 200 400 A2 1 99 200 400 0.05 АЗ 2 200 400 0.10 A4 4 4 200 400 0.25 A5 6. 6. 200 400 0.3 A6 8 200 400 0.38 A7 10 200 400 0.55 A8 20 200 400 0.77 Table 2: Calculation of volumes to add for experiment
4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400 µL in each well. Calculate the volumes of each reagent that needs to be added and complete the table below (fill in the blue boxes). [BSA] µg/ BSA (µL) Bradford Total volume Absorbance Well Water (µL) Reagent (µL) OD 595nm (A595) (µL) A1 100 200 400 A2 1 99 200 400 0.05 АЗ 2 200 400 0.10 A4 4 4 200 400 0.25 A5 6. 6. 200 400 0.3 A6 8 200 400 0.38 A7 10 200 400 0.55 A8 20 200 400 0.77 Table 2: Calculation of volumes to add for experiment
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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I just need help with line A7 across.
![4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400
µL in each well. Calculate the volumes of each reagent that needs to be added and complete the
table below (fill in the blue boxes).
[BSA] µg/ BSA (µL)
Bradford
Total volume
Absorbance
Well
Water
(µL)
Reagent
(µL)
OD 595nm (A595)
(µL)
A1
100
200
400
A2
1
99
200
400
0.05
АЗ
2
200
400
0.10
A4
4
4
200
400
0.25
A5
6.
6.
200
400
0.3
A6
8
200
400
0.38
A7
10
200
400
0.55
A8
20
200
400
0.77
Table 2: Calculation of volumes to add for experiment](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9833fd88-36f3-47db-935b-63b09cc944cc%2F59d9a941-b03e-4990-b7b8-024bf66ee836%2Fkqt7fer.jpeg&w=3840&q=75)
Transcribed Image Text:4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400
µL in each well. Calculate the volumes of each reagent that needs to be added and complete the
table below (fill in the blue boxes).
[BSA] µg/ BSA (µL)
Bradford
Total volume
Absorbance
Well
Water
(µL)
Reagent
(µL)
OD 595nm (A595)
(µL)
A1
100
200
400
A2
1
99
200
400
0.05
АЗ
2
200
400
0.10
A4
4
4
200
400
0.25
A5
6.
6.
200
400
0.3
A6
8
200
400
0.38
A7
10
200
400
0.55
A8
20
200
400
0.77
Table 2: Calculation of volumes to add for experiment
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