Trypsin treatment decreases oxidative phosphorylation

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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In your final experiment, your goal is to study the correlation between ADP permeability and
mitochondrial distribution.
The following bar graph shows the Km (ADP) measured after cells were treated for 5 minutes with
trypsin.
350
O uM
300
250
200
150
0.3 uM
0.5 µM
100
1uM
O uM
50
1
2
4
The red bars correspond to the Km (ADP) of cardiomyocytes treated with increasing concentration
of trypsin (0 to 1 mM), the blue bar represents the Km(ADP) of untreated HL-1 cells.
Based on these data and the results of previous experiments, what following statements are true
(T) or false (F)?
Trypsin treatment decreases oxidative phosphorylation
O True
O False
Trypsin treatment increases ADP permeability of mitochondria
O True
O False
The distribution of mitochondria into untreated HL-1 cells and cardiomyocytes are similar
O True
O False
Transcribed Image Text:In your final experiment, your goal is to study the correlation between ADP permeability and mitochondrial distribution. The following bar graph shows the Km (ADP) measured after cells were treated for 5 minutes with trypsin. 350 O uM 300 250 200 150 0.3 uM 0.5 µM 100 1uM O uM 50 1 2 4 The red bars correspond to the Km (ADP) of cardiomyocytes treated with increasing concentration of trypsin (0 to 1 mM), the blue bar represents the Km(ADP) of untreated HL-1 cells. Based on these data and the results of previous experiments, what following statements are true (T) or false (F)? Trypsin treatment decreases oxidative phosphorylation O True O False Trypsin treatment increases ADP permeability of mitochondria O True O False The distribution of mitochondria into untreated HL-1 cells and cardiomyocytes are similar O True O False
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