Be sure to answer all parts.Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems.(K, = 5.01 x 109 and pk, 8.3)%3D%3D(a) Calculate the pH of the Tris buffer after mixing 10.5 mL of 0.20 M HCI solution with 25.0 mL of0.10 M Tris.7.06(b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00020mol of H* was produced. What is the pH of the buffer at the end of the reaction?(c) What would be the final pH if no buffer were present?

Molarity is a measure of the concentration of a solution, expressed as the number of moles of solute…

Answered: Tris [tris(hydroxymethyl)aminomethane]…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The structure of ephedrine isgiven, The pKa for ephedrine is 9.6. Answer the following questions about aqueous solutions of ephedrine. a. Draw the structure of the principal chemical form of ephedrine that results when it isdissolved in water.b. Draw the structure of the principal chemical form of ephedrine that results when it is dissolved in a buffer at a physiologically relevant pH of 7.4.c. Calculate the pH that results when 0.3 mole of ephedrine is dissolve in 2.0 L of 0.05 M perchloric acid (HClO4).
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TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE buffer and you have the following stock solutions: 500 mM Tris-Cl and 1 M EDTA. How will you prepare the 3L of TE? Write work clearly
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the enzyme LCK catalyzes the hydrolysis of the peptide KEY in 5.0 mM phosphate buffer at pH 7.0. The enzyme is known to obey Michaelis-Menten Kinetics. Under the conditions of this experiment: Vmax= 10 mmol/min/mg Km (KEY) = 0.20 mM Draw the complete structure of the tripeptide KEY and calculate its molecular weight Sketch the titration curve of the above tripeptide and label it.Determine the net charge of the peptide at each pk value Calculate the PI pf the peptide and draw the structure of the peptide at the isoelectric point.
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The pKa values of a compound with two ionizable groups are pK1 = 4.10 and pK2 between 7 and 10. A biochemist has 10 mL of a 1.0 M solution of this compound at a pH of 8.00. She adds 10.0 mL of 1.00 M HCl, which changes the pH to 3.20. What is pK2?
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