Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which can be used for the spectrophotometric determination of ammonia. Ocr 2 OH + NH3 O: indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 × 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) А 0.543 В 0.790 C 0.045 What is the molar absorptivity (e) of the indophenol product? € = M-'cm-! What is the concentration of ammonia in the lake water? [NH, Jjake water M
Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which can be used for the spectrophotometric determination of ammonia. Ocr 2 OH + NH3 O: indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 × 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) А 0.543 В 0.790 C 0.045 What is the molar absorptivity (e) of the indophenol product? € = M-'cm-! What is the concentration of ammonia in the lake water? [NH, Jjake water M
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at
625 nm, which can be used for the spectrophotometric determination of ammonia.
Ocr
2
OH + NH3
O:
indophenol anion
To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake
water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric
flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and
2.50 mL of a 5.50 × 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of
distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the
three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table.
Sample Absorbance (625 nm)
А
0.543
В
0.790
C
0.045
What is the molar absorptivity (e) of the indophenol product?
€ =
M-'cm-!
What is the concentration of ammonia in the lake water?
[NH, Jjake water
M](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F83ae3fb9-1c11-4f71-8a0d-474834ecd625%2Ff637fadf-9787-4713-8434-0fb5b05e8592%2Fx7g80f7_processed.png&w=3840&q=75)
Transcribed Image Text:Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at
625 nm, which can be used for the spectrophotometric determination of ammonia.
Ocr
2
OH + NH3
O:
indophenol anion
To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake
water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric
flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and
2.50 mL of a 5.50 × 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of
distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the
three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table.
Sample Absorbance (625 nm)
А
0.543
В
0.790
C
0.045
What is the molar absorptivity (e) of the indophenol product?
€ =
M-'cm-!
What is the concentration of ammonia in the lake water?
[NH, Jjake water
M
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