Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which can be used for the spectrophotometric determination of ammonia. ocr -OH + NH, indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 ml. of lake water is mixed with 5 mL of phenol solution and 2 ml. of sodium hypochlorite solution, and diluted to 25.0 ml in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 ml of a 5.50 x 10 M ammonia solution, and diluted to 25.0 ml. Sample Cis a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 ml. of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) 0.486 B 0.733 0.045 What is the molar absorptivity (c) of the indophenol product? M-'em What is the concentration of ammonia in the lake water? (NH,Juke water
Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which can be used for the spectrophotometric determination of ammonia. ocr -OH + NH, indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 ml. of lake water is mixed with 5 mL of phenol solution and 2 ml. of sodium hypochlorite solution, and diluted to 25.0 ml in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 ml of a 5.50 x 10 M ammonia solution, and diluted to 25.0 ml. Sample Cis a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 ml. of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) 0.486 B 0.733 0.045 What is the molar absorptivity (c) of the indophenol product? M-'em What is the concentration of ammonia in the lake water? (NH,Juke water
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at
625 nm, which can be used for the spectrophotometric determination of ammonia.
ocr
-OH + NH,
indophenol anion
To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake
water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric
flask. In sample B, 10.0 ml. of lake water is mixed with 5 ml of phenol solution, 2 mL of sodium hypochlorite solution, and
2.50 mL of a 5.50 x 10 M ammonia solution, and diluted to 25.0 mL. Sample Cis a reagent blank. It contains 10.0 mL of
distilled water, 5 mL of phenol solution, and 2 mL. of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the
three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table.
Sample Absorbance (625 nm)
A
0.486
в
0.733
0.045
What is the molar absorptivity (e) of the indophenol product?
M-'em
What is the concentration of ammonia in the lake water?
M
(NH,Juke water
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