Total plasma volume is important in determining the required plasma component in blood replacement therapy for a person undergoing surgery. Plasma volume is influenced by the overall health and  physical activity of the individual. Suppose that a random sample of 45 male firefighters are tested and that they have a plasma volume sample mean of x bar = 37.5 ml/kg. Assume that d standard deviation = 7.50 ml/kg for the distribution of blood plasma. (d) Sample size. Find the sample size necessary for a 99% confidence level with maximal margin of error E=2.50 for the mean plasma volume in male firefighters.

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Statistics question - I need help with part (d).

Total plasma volume is important in determining the required plasma component in blood replacement therapy for a person undergoing surgery. Plasma volume is influenced by the overall health and  physical activity of the individual. Suppose that a random sample of 45 male firefighters are tested and that they have a plasma volume sample mean of x bar = 37.5 ml/kg. Assume that d standard deviation = 7.50 ml/kg for the distribution of blood plasma.

(d) Sample size. Find the sample size necessary for a 99% confidence level with maximal margin of error E=2.50 for the mean plasma volume in male firefighters.

I know the answer is n=60 but I don't know how that was found. Can you please explain formula used/show work? Thanks.

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