To investigate the properties of cell membranes, a scientist used the Fluorescence Recovery After Photobleaching (FRAP) technique. Based on this method, all the membrane proteins on the cell surface are to be uniformly labeled with a green fluorescent dye. After 10 minutes, a small area on the cell membrane will be photo-bleached by shining a powerful laser at it (this laser permanently destroys the fluorescent molecules in the area). The fluorescence in the photobleached area will be measured over time. Which of the below statement/s is supported by this observation? (Mark all that apply/more than one answer is accepted) Nucleoli Nucleus containing GFP-POL Prebleach T-O sec a Laser bleaching T-3 sec 0 Fluorescence recovery after bleaching T-3 sec until end of measurement 50 100 150 Time[sec] 200 250 300 O a. The cell membrane is fluid, allowing proteins from adjacent areas to move into the photobleached area O b. New proteins are synthesized in the cell and transported to the cell surface O c. Fluorescent molecules are not permanently destroyed by the laser beam. They can spontaneously regain their fluorescence over time. Od. The rate of fluorescent recovery is directly related to the rate of protein diffusion
To investigate the properties of cell membranes, a scientist used the Fluorescence Recovery After Photobleaching (FRAP) technique. Based on this method, all the membrane proteins on the cell surface are to be uniformly labeled with a green fluorescent dye. After 10 minutes, a small area on the cell membrane will be photo-bleached by shining a powerful laser at it (this laser permanently destroys the fluorescent molecules in the area). The fluorescence in the photobleached area will be measured over time. Which of the below statement/s is supported by this observation? (Mark all that apply/more than one answer is accepted) Nucleoli Nucleus containing GFP-POL Prebleach T-O sec a Laser bleaching T-3 sec 0 Fluorescence recovery after bleaching T-3 sec until end of measurement 50 100 150 Time[sec] 200 250 300 O a. The cell membrane is fluid, allowing proteins from adjacent areas to move into the photobleached area O b. New proteins are synthesized in the cell and transported to the cell surface O c. Fluorescent molecules are not permanently destroyed by the laser beam. They can spontaneously regain their fluorescence over time. Od. The rate of fluorescent recovery is directly related to the rate of protein diffusion
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
asap please.
![To investigate the properties of cell membranes, a scientist used the Fluorescence Recovery After
Photobleaching (FRAP) technique. Based on this method, all the membrane proteins on the cell
surface are to be uniformly labeled with a green fluorescent dye. After 10 minutes, a small area on
the cell membrane will be photo-bleached by shining a powerful laser at it (this laser permanently
destroys the fluorescent molecules in the area). The fluorescence in the photobleached area will be
measured over time. Which of the below statement/s is supported by this observation?
(Mark all that apply/more than one answer is accepted)
Nucleoli Nucleus containing
GFP-POI
Prebleach
T-O sec
S
Laser bleaching
T-3 sec
0
50
Fluorescence recovery after bleaching
T-3 sec until end of measurement
100 150 200 250 300
Time[sec]
O a. The cell membrane is fluid, allowing proteins from adjacent areas to move into the photobleached area
O b. New proteins are synthesized in the cell and transported to the cell surface
O c. Fluorescent molecules are not permanently destroyed by the laser beam. They can spontaneously regain
their fluorescence over time.
Od. The rate of fluorescent recovery is directly related to the rate of protein diffusion](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa28cb2d5-bdf9-4a0e-bcb5-4f5025e068fb%2Fd644fbd9-0daa-4bba-a255-b044ed1a6dae%2Fowfmab_processed.png&w=3840&q=75)
Transcribed Image Text:To investigate the properties of cell membranes, a scientist used the Fluorescence Recovery After
Photobleaching (FRAP) technique. Based on this method, all the membrane proteins on the cell
surface are to be uniformly labeled with a green fluorescent dye. After 10 minutes, a small area on
the cell membrane will be photo-bleached by shining a powerful laser at it (this laser permanently
destroys the fluorescent molecules in the area). The fluorescence in the photobleached area will be
measured over time. Which of the below statement/s is supported by this observation?
(Mark all that apply/more than one answer is accepted)
Nucleoli Nucleus containing
GFP-POI
Prebleach
T-O sec
S
Laser bleaching
T-3 sec
0
50
Fluorescence recovery after bleaching
T-3 sec until end of measurement
100 150 200 250 300
Time[sec]
O a. The cell membrane is fluid, allowing proteins from adjacent areas to move into the photobleached area
O b. New proteins are synthesized in the cell and transported to the cell surface
O c. Fluorescent molecules are not permanently destroyed by the laser beam. They can spontaneously regain
their fluorescence over time.
Od. The rate of fluorescent recovery is directly related to the rate of protein diffusion
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 4 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education