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To further understand lysosomal function, you have created mouse cell lines, each with a different lysosomal defect. The first line, A1, synthesizes lysosomal enzymes that are perfectly functional, but they are not addressed properly to go to the lysosome and instead are secreted outside the cell. The other line, B2, has a mutation in a single lysosomal phospholipase, so their lysosomes cannot degrade certain classes of phospholipids. Which of the following experimental outcomes is consistent with the above information? Culture medium from neither mouse line can correct the defect in the other. Cell culture medium from A1 contains functional lysosomal enzymes, which will rescue the defects in B2. Cell culture medium from A1 contains defective lysosomal enzymes, which will cause a dominant negative effect on B2 cells. Culture medium taken from each mouse line can correct the defect in cells of the other mouse lines, so cells from both lines can grow.

To further understand lysosomal function, you have created mouse cell lines, each with a different lysosomal defect. The first line, A1, synthesizes lysosomal enzymes that are perfectly functional, but they are not addressed properly to go to the lysosome and instead are secreted outside the cell. The other line, B2, has a mutation in a single lysosomal phospholipase, so their lysosomes cannot degrade certain classes of phospholipids. Which of the following experimental outcomes is consistent with the above information? Culture medium from neither mouse line can correct the defect in the other. Cell culture medium from A1 contains functional lysosomal enzymes, which will rescue the defects in B2. Cell culture medium from A1 contains defective lysosomal enzymes, which will cause a dominant negative effect on B2 cells. Culture medium taken from each mouse line can correct the defect in cells of the other mouse lines, so cells from both lines can grow.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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To further understand lysosomal function, you have created mouse cell lines, each with a different lysosomal defect. The first line, A1, synthesizes lysosomal enzymes that are perfectly functional, but they are not addressed properly to go to the lysosome and instead are secreted outside the cell. The other line, B2, has a mutation in a single lysosomal phospholipase, so their lysosomes cannot degrade certain classes of phospholipids. Which of the following experimental outcomes is consistent with the above information? Culture medium from neither mouse line can correct the defect in the other. O Cell culture medium from A1 contains functional lysosomal enzymes, which will rescue the defects in B2. Cell culture medium from A1 contains defective lysosomal enzymes, which will cause a dominant negative effect on B2 cells. O Culture medium taken from each mouse line can correct the defect in cells of the other mouse lines, so cells from both lines can grow.
Transcribed Image Text:To further understand lysosomal function, you have created mouse cell lines, each with a different lysosomal defect. The first line, A1, synthesizes lysosomal enzymes that are perfectly functional, but they are not addressed properly to go to the lysosome and instead are secreted outside the cell. The other line, B2, has a mutation in a single lysosomal phospholipase, so their lysosomes cannot degrade certain classes of phospholipids. Which of the following experimental outcomes is consistent with the above information? Culture medium from neither mouse line can correct the defect in the other. O Cell culture medium from A1 contains functional lysosomal enzymes, which will rescue the defects in B2. Cell culture medium from A1 contains defective lysosomal enzymes, which will cause a dominant negative effect on B2 cells. O Culture medium taken from each mouse line can correct the defect in cells of the other mouse lines, so cells from both lines can grow.
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