Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated by overnight digestion in 5 mL of 0.3 % collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks. The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable. What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)? Number How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹ Number In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]? * Number What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1 decimal place? [ Number

Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated by overnight digestion in 5 mL of 0.3 % collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks. The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable. What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)? Number How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹ Number In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]? * Number What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1 decimal place? [ Number

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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