These are the results from my experiment of determining the optimal temperature for amylase. The question I have is what is the optimal temperature of the reaction? (Would it be 55 degrees Celsius?) and at what temperature does amylase denature...my guess is 65 degrees
These are the results from my experiment of determining the optimal temperature for amylase. The question I have is what is the optimal temperature of the reaction? (Would it be 55 degrees Celsius?) and at what temperature does amylase denature...my guess is 65 degrees
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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These are the results from my experiment of determining the optimal temperature for amylase. The question I have is what is the optimal temperature of the reaction? (Would it be 55 degrees Celsius?) and at what temperature does amylase denature...my guess is 65 degrees
Transcribed Image Text:**Enzymatic Activity**
The table below represents the results of the experiment, including the average time of reaction completion for each of the enzyme concentrations tested.
| Temperature of Reactions | Average Time to Completion (seconds) | Notes on Progress of Reaction |
|--------------------------|--------------------------------------|----------------------------------------------------|
| 5°C | >540 | Reaction appears almost 80% complete |
| 15°C | 330 | |
| 25°C | 225 | |
| 35°C | 90 | |
| 45°C | 75 | |
| 55°C | 60 | |
| 65°C | >540 | Reaction appears about 50% complete |
| 75°C | >540 | No reaction has occurred |
| 85°C | >540 | No reaction has occurred |
This table illustrates the effect of temperature on the average time taken for reactions to reach completion. It shows varying levels of reaction progress across different temperatures, indicating optimal conditions for enzymatic activity. At lower and higher temperatures, reactions are less complete or do not occur.
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