The steady state kinetics of an enzyme was studied in the absence and presence of an inhibitor A. The initial velocities are given as a function of substrate concentration in the following table. Carefully construct the Lineweaver-Burk Plot on graph paper be sure to plot all data points (Excel Graphs earn zero points, hand drawn graphs on notebook paper earns zero points). No Inhibitor 175.50 μM Inhibitor A V μM/s [S] μη 2.00 2.50 3.33 5.00 10.00 Calculate K; for this Inhibitor Report your answer in M 6.667 7.692 9.091 11.111 14.286 V μM/s 1.818 2.000 2.222 2.500 2.857

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
icon
Related questions
Question

help pls with how to calculate 

29
The steady state kinetics of an enzyme was studied in the absence and presence of an inhibitor A. The initial velocities are given as a function of
substrate concentration in the following table. Carefully construct the Lineweaver-Burk Plot on graph paper be sure to plot all data points (Excel
Graphs earn zero points, hand drawn graphs on notebook paper earns zero points).
No Inhibitor
175.50 μM Inhibitor A
V
μM/s
6.667
7.692
9.091
11.111
14.286
[S]
μη
2.00
2.50
3.33
5.00
10.00
Calculate K; for this Inhibitor
Report your answer in μM
V
μM/s
1.818
2.000
2.222
2.500
2.857
---D-O
Transcribed Image Text:29 The steady state kinetics of an enzyme was studied in the absence and presence of an inhibitor A. The initial velocities are given as a function of substrate concentration in the following table. Carefully construct the Lineweaver-Burk Plot on graph paper be sure to plot all data points (Excel Graphs earn zero points, hand drawn graphs on notebook paper earns zero points). No Inhibitor 175.50 μM Inhibitor A V μM/s 6.667 7.692 9.091 11.111 14.286 [S] μη 2.00 2.50 3.33 5.00 10.00 Calculate K; for this Inhibitor Report your answer in μM V μM/s 1.818 2.000 2.222 2.500 2.857 ---D-O
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps with 5 images

Blurred answer
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The