The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 1 2 0.2 .063 3 0.4 163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 excellent data; r2 0.98-0.95 good data r2 0.94-0.90 = fair data. How would you describe this data?-

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Chapter1: Chemical Foundations
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The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is
shown below: Cuvette 1 is the clank
Cuvette #
ml albumin
Absorbance
0.
0.2
.063
3
0.4
.163
4.
0.6
.187
0.8
.253
1.0
.289
Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the
correlation coefficient (r). R2 > 0.99 = excellent data; r2 0.98-0.95 good data r2 0.94 -0.90 =
fair data. How would you describe this data?-
To find the relative sensitivity, for each of tubes 2-6 (neglect blank), perform the following
operation absorbance (Lowry)/Absorbance (Biuret x 0.5 gram albumin (Biuret)/0.05 gram
(Lowry) x 200 ml (Lowry)/100 mL (Biuret). This implifies to Relative sensitivity = 20 dilution
factor x absorbance (Lowry)/Absorbance (Biuret). Report your relative sensitivity as an average
of all 5 values.
Transcribed Image Text:The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 0. 0.2 .063 3 0.4 .163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 = excellent data; r2 0.98-0.95 good data r2 0.94 -0.90 = fair data. How would you describe this data?- To find the relative sensitivity, for each of tubes 2-6 (neglect blank), perform the following operation absorbance (Lowry)/Absorbance (Biuret x 0.5 gram albumin (Biuret)/0.05 gram (Lowry) x 200 ml (Lowry)/100 mL (Biuret). This implifies to Relative sensitivity = 20 dilution factor x absorbance (Lowry)/Absorbance (Biuret). Report your relative sensitivity as an average of all 5 values.
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