The sequence shown below is an actual sequence of the toxin gene from Salmonella enterica subspecies serovar Typhimurium strain obtained from the NCBI database. ATGCGAACCTTCAAAACCAGGTGGTTTAACAGAGAAGCGAAGCCCCACACGATAAAAGATGACGAGTTAA GCGAGGCCATCAACGCCGTACTGCAAGGAAAAGCAGATAATCTTGGCGGCGGGGTTTATAAAAAACGTCT CAATCAAAATCGCGATCGCGCAATCGTGTTGGCAAAGGGAGGCGAACATTGGTTTTACACCTTCCTGTAT GCCAAACAGGATATGGCCAACATTAGCTATCGCGAACTCGCGGGTTTCCGTGAGTTAGCAAAACACTATG CTTGCCTGACCGAAGATCAGATAACGGCACTCATTAATAACAAAGAACTGGTAGAGGTGCGCCATGTCAG CAAAAACTAA (i) Is the nucleotide sequence above translatable into a complete protein? Give justification. (ii) Is the sequence above represented in the standard FASTA format? Explain your answer. (iii) Liliana, a research officer would like to perform a multiple sequence alignment between the sequence above with eight other toxin genes sequences obtained from other researchers. Define multiple sequence alignment. (iv) Which multiple sequence alignment program would you recommend to Liliana for the comparison of the toxin sequence above with other toxin genes from other organisms?

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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The sequence shown below is an actual sequence of the toxin gene from Salmonella
enterica subspecies serovar Typhimurium strain obtained from the NCBI database.
ATGCGAACCTTCAAAACCAGGTGGTTTAACAGAGAAGCGAAGCCCCACACGATAAAAGATGACGAGTTAA
GCGAGGCCATCAACGCCGTACTGCAAGGAAAAGCAGATAATCTTGGCGGCGGGGTTTATAAAAAACGTCT
CAATCAAAATCGCGATCGCGCAATCGTGTTGGCAAAGGGAGGCGAACATTGGTTTTACACCTTCCTGTAT
GCCAAACAGGATATGGCCAACATTAGCTATCGCGAACTCGCGGGTTTCCGTGAGTTAGCAAAACACTATG
CTTGCCTGACCGAAGATCAGATAACGGCACTCATTAATAACAAAGAACTGGTAGAGGTGCGCCATGTCAG
CAAAAACTAA
(i)
Is the nucleotide sequence above translatable into a complete protein? Give justification.
(ii) Is the sequence above represented in the standard FASTA format? Explain your answer.
(iii) Liliana, a research officer would like to perform a multiple sequence alignment between
the sequence above with eight other toxin genes sequences obtained from other
researchers. Define multiple sequence alignment.
(iv) Which multiple sequence alignment program would you recommend to Liliana for the
comparison of the toxin sequence above with other toxin genes from other organisms?
Transcribed Image Text:The sequence shown below is an actual sequence of the toxin gene from Salmonella enterica subspecies serovar Typhimurium strain obtained from the NCBI database. ATGCGAACCTTCAAAACCAGGTGGTTTAACAGAGAAGCGAAGCCCCACACGATAAAAGATGACGAGTTAA GCGAGGCCATCAACGCCGTACTGCAAGGAAAAGCAGATAATCTTGGCGGCGGGGTTTATAAAAAACGTCT CAATCAAAATCGCGATCGCGCAATCGTGTTGGCAAAGGGAGGCGAACATTGGTTTTACACCTTCCTGTAT GCCAAACAGGATATGGCCAACATTAGCTATCGCGAACTCGCGGGTTTCCGTGAGTTAGCAAAACACTATG CTTGCCTGACCGAAGATCAGATAACGGCACTCATTAATAACAAAGAACTGGTAGAGGTGCGCCATGTCAG CAAAAACTAA (i) Is the nucleotide sequence above translatable into a complete protein? Give justification. (ii) Is the sequence above represented in the standard FASTA format? Explain your answer. (iii) Liliana, a research officer would like to perform a multiple sequence alignment between the sequence above with eight other toxin genes sequences obtained from other researchers. Define multiple sequence alignment. (iv) Which multiple sequence alignment program would you recommend to Liliana for the comparison of the toxin sequence above with other toxin genes from other organisms?
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The process of DNA sequencing is used to ascertain the nucleotide sequence of deoxyribonucleic acid. The most fundamental understanding of a gene or genome is its nucleotide sequence. 

Without having this knowledge, it is impossible to fully comprehend how genes function or how organisms evolve. It is the blueprint that holds the instructions for developing an organism.

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