The separation of molecules within a gel is determined by the relative size of the pores formed within the gel. Thus, the gel percentage depends on the size of your protein of interest. Protein size (kDa) Gel percentage (%) 4-40 20 12-45 15 10-70 12.5 15-100 10 25-200 8
The separation of molecules within a gel is determined by the relative size of the pores formed within the gel. Thus, the gel percentage depends on the size of your protein of interest. Protein size (kDa) Gel percentage (%) 4-40 20 12-45 15 10-70 12.5 15-100 10 25-200 8
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
For leptin, what gel percentage are you going to prepare?
![The separation of molecules within a gel is determined by the relative size of the pores
formed within the gel. Thus, the gel percentage depends on the size of your protein of
interest.
Protein size (kDa)
Gel percentage (%)
4-40
20
12-45
15
10-70
12.5
15-100
10
25-200
8](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd1ab8d46-4a82-4032-afaa-ceab24d5a35b%2Fd35a344a-7d72-4c95-bb7d-2f297e493eb4%2Fbklfv8y_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The separation of molecules within a gel is determined by the relative size of the pores
formed within the gel. Thus, the gel percentage depends on the size of your protein of
interest.
Protein size (kDa)
Gel percentage (%)
4-40
20
12-45
15
10-70
12.5
15-100
10
25-200
8
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may I ask how you get 12-65 of the leptin's size?
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