The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Solutions

Solutions can be considered as homogeneous mixtures which contain two or more than two chemical substances. The chemical substance which is the greater part is defined as solvent. And the chemical substance that is dissolved in a solvent is referred to as solute. Solution chemistry plays an important role in chemistry since it supports many commercial applications of solutions.

Question

Aa.17.

The relative polarities of a few common solvents are given below:
Relative polarity
Solvent name
Water
Acetic acid
Acetonitrile
CO II
Ol
IV
1
While using a C-18 hydrocarbon column for protein purification, which of the following will
be the best elution solvent mixture? The final polarity of the solvent mixture can be
predicted by considering the relative % of each constituent and their respective relative
polarity values provide.
1. 90% water and 10% acetic acid
II. 90% acetic acid and 10% acetonitrile
III. 90% acetonitrile and 10% water
IV. 10% acetonitrile and 90% acetic acid
|||
0.65
0.48

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