The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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