The portion (the C-terminal end) of original substrate with the new amino terminus diffuses away. His 57 catalyzes removal of H from Ser 195 hydroxyl. Water donates H to His 57. The portion (N-terminal end) of original substrate with the new carboxylate terminus diffuses away. Ser 195's nucleophilic O attacks carbonyl C of substrate. His 57 donates H to N of scissile peptide bond, tetrahedral intermediate decomposes. His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate. Resulting OH attacks carbonyl of remaining substrate.
The portion (the C-terminal end) of original substrate with the new amino terminus diffuses away. His 57 catalyzes removal of H from Ser 195 hydroxyl. Water donates H to His 57. The portion (N-terminal end) of original substrate with the new carboxylate terminus diffuses away. Ser 195's nucleophilic O attacks carbonyl C of substrate. His 57 donates H to N of scissile peptide bond, tetrahedral intermediate decomposes. His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate. Resulting OH attacks carbonyl of remaining substrate.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Answer Bank
The portion (the C-terminal end) of original substrate with the new amino terminus diffuses away.
His 57 catalyzes removal of H from Ser 195 hydroxyl.
Water donates H to His 57.
The portion (N-terminal end) of original substrate with the new carboxylate terminus diffuses away.
Ser 195's nucleophilic O attacks carbonyl C of substrate.
His 57 donates H to N of scissile peptide bond, tetrahedral intermediate decomposes.
His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate.
Resulting OH attacks carbonyl of remaining substrate.
Transcribed Image Text:Put these steps in the mechanism of chymotrypsin catalysis in order from first to last. Note that some references may not break
out each of these steps individually, but all steps should be ordered. There will be eight steps.
First step
Last step
Answer Bank
The portion (the C-terminal end) of original substrate with the new amino terminus diffuses away.
His 57 catalyzes removal of H from Ser 195 hydroxyl.
Water donates H to His 57.
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