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Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Please help urgently with the complete answer of this question asap
MRGSHHHHHHGSVSKGEELFTGVVPILVELDGDVN
GHKFSVSGEGEGDATYGKLTLKFIC
MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD
ATYGKLTLKFIC
Note these are incomplete sequences.
Is there any purpose in the different amino acid
sequence at the start (other than the
polyhistideine tag)? Why is the recombinant
protein amino acid 12 aa longer when the histidine
tag is only 6 aa long?
Transcribed Image Text:MRGSHHHHHHGSVSKGEELFTGVVPILVELDGDVN GHKFSVSGEGEGDATYGKLTLKFIC MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD ATYGKLTLKFIC Note these are incomplete sequences. Is there any purpose in the different amino acid sequence at the start (other than the polyhistideine tag)? Why is the recombinant protein amino acid 12 aa longer when the histidine tag is only 6 aa long?
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