The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. Vmax [S] Km + [S] where u is the velocity, or rate, Vmax is the maximum velocity, K., is the Michaelis Menten constant, and [S] is the substrate concentration. A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (U) at different substrate concentrations ([S]). V= First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. Next, move the line labeled 1/2 Vmax to its correct position. Then, move the line labeled Km to its correct position. Estimate the values for Vmax and Km. V max = µM/min v (µM/min) 300 275 250 225 200 175 150 125 100 Km = 75 50 25 0 K m 0 10 20 30 V max 40 50 [S] (μM) 1/2 V 60 Michaelis-Menten curve max 70 80 90 100 μM

The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. Vmax [S] Km + [S] where u is the velocity, or rate, Vmax is the maximum velocity, K., is the Michaelis Menten constant, and [S] is the substrate concentration. A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (U) at different substrate concentrations ([S]). V= First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. Next, move the line labeled 1/2 Vmax to its correct position. Then, move the line labeled Km to its correct position. Estimate the values for Vmax and Km. V max = µM/min v (µM/min) 300 275 250 225 200 175 150 125 100 Km = 75 50 25 0 K m 0 10 20 30 V max 40 50 [S] (μM) 1/2 V 60 Michaelis-Menten curve max 70 80 90 100 μM

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Question 2
Question 47 Translation is_ : O Use of RNA as a template to make proteins O Use of DNA as a template to make complementary DNA O Use of DNA as a template to make RNA
The genetic code is identical in cyanobacteria, lions, and humans Question 25 options: True False
Question 15
Please expound your answer. If you'll write, please do it legibly and clearly. Thank you. Explain the significance of Wohler’s synthesis of urea in his lab.
Question 2
Question 43 Nucleotide triphosphates and nucleotide diphosphates often form stable complexes with Na Fe3 Mg2 K* Question 46
Question 6 Milligram of base required to completely hydrolyze & neutralize 1 g of triglyceride/fat sample Ester number Saponification number O Acid number O lodine number
Question 3a and 3c.
This test can be saved and resumed later. The timer will continue to run if you leave the test. Completion Your answers are saved automatically. Remaining Trime: 1 hour, 09 minutes, 43 seconds. Question Completion Status: A Moving to another question will save this response. Question 13 True/False Question (suggested time - up to 1 minute): Since there are 20 proteinogenic amino acids, most cells contain at least 20 aminoacyl-tRNA synthetases (one enzyme for each amino acid). O True O False A Moving to another question wili save this response. Iyp
Question Number-1: Using complementary base pairing method, convert the following DNA sequence into RNA sequence. DNA: TACGGCGCGTATACGACT
i need help with these questions! please and thank you a. When would you use Edman degradation method and mass spectrometry inprotein sequencing experiment?b. What is the biochemical information you acquire from amino acid sequence?Give three examples.c. You are say buying a pure enzyme for your experiment. Would you choose an enzyme with high activity or high specific activity? Why/