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The generation of a transgenic plant Engineered T-DNA Tobacco-plant cell T-DNA inserts into a plant chromosome Transformed cell kan cells selected Transgenic Cultured cells Cell of transgenic plant tobacco Plantlet plant FIGURE 10-25 The insertion of T-DNA into plant chromosomes. Incubation of leaf disks with the bacterium A. tumefaciens plasmid. When the bacterium infects a plant cell, a part of the Ti plasmid is trans- ferred and inserted, apparently more or less at random, into the genome of the host plant (Figure 10-24). The region of the Ti plasmid that inserts into the host plant is called T-DNA, for transfer DNA. The genes whose products catalyze this T-DNA transfer reside in a region of the Ti plasmid separate from the T-DNA region itself. The natural behavior of the Ti plasmid makes it well suited to the role of a vector for plant genetic engineering. In particular, any DNA that is inserted between the T-DNA border (24-bp ends) sequences can be mobilized by other functions pro- vided by the Ti plasmid and inserted into plant chromosomes. Thus, scientists were able to eliminate all of the T-DNA sequence between the borders (including the tumor-causing genes) and replace it with the gene(s) of interest and a selectable marker (for example, kanamycin resistance). One method of introducing the T-DNA containing an engineered T-DNA leads to leaf cells with the T-DNA in their genome, which are able to grow on agar plates and can be coaxed to differentiate into transgenic tobacco plants. un 10 25 imi

The generation of a transgenic plant Engineered T-DNA Tobacco-plant cell T-DNA inserts into a plant chromosome Transformed cell kan cells selected Transgenic Cultured cells Cell of transgenic plant tobacco Plantlet plant FIGURE 10-25 The insertion of T-DNA into plant chromosomes. Incubation of leaf disks with the bacterium A. tumefaciens plasmid. When the bacterium infects a plant cell, a part of the Ti plasmid is trans- ferred and inserted, apparently more or less at random, into the genome of the host plant (Figure 10-24). The region of the Ti plasmid that inserts into the host plant is called T-DNA, for transfer DNA. The genes whose products catalyze this T-DNA transfer reside in a region of the Ti plasmid separate from the T-DNA region itself. The natural behavior of the Ti plasmid makes it well suited to the role of a vector for plant genetic engineering. In particular, any DNA that is inserted between the T-DNA border (24-bp ends) sequences can be mobilized by other functions pro- vided by the Ti plasmid and inserted into plant chromosomes. Thus, scientists were able to eliminate all of the T-DNA sequence between the borders (including the tumor-causing genes) and replace it with the gene(s) of interest and a selectable marker (for example, kanamycin resistance). One method of introducing the T-DNA containing an engineered T-DNA leads to leaf cells with the T-DNA in their genome, which are able to grow on agar plates and can be coaxed to differentiate into transgenic tobacco plants. un 10 25 imi

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In Figure 10-25, why do only plant cells that have T-DNA
inserts in their chromosomes grow on the agar plates?
Do all of the cells of a transgenic plant grown from one
clump of cells contain T-DNA? Justify your answer

The generation of a transgenic plant Engineered T-DNA Tobacco-plant cell T-DNA inserts into a plant chromosome Transformed cell kan cells selected Transgenic Cultured cells Cell of transgenic plant tobacco Plantlet plant FIGURE 10-25 The insertion of T-DNA into plant chromosomes. Incubation of leaf disks with the bacterium A. tumefaciens plasmid. When the bacterium infects a plant cell, a part of the Ti plasmid is trans- ferred and inserted, apparently more or less at random, into the genome of the host plant (Figure 10-24). The region of the Ti plasmid that inserts into the host plant is called T-DNA, for transfer DNA. The genes whose products catalyze this T-DNA transfer reside in a region of the Ti plasmid separate from the T-DNA region itself. The natural behavior of the Ti plasmid makes it well suited to the role of a vector for plant genetic engineering. In particular, any DNA that is inserted between the T-DNA border (24-bp ends) sequences can be mobilized by other functions pro- vided by the Ti plasmid and inserted into plant chromosomes. Thus, scientists were able to eliminate all of the T-DNA sequence between the borders (including the tumor-causing genes) and replace it with the gene(s) of interest and a selectable marker (for example, kanamycin resistance). One method of introducing the T-DNA containing an engineered T-DNA leads to leaf cells with the T-DNA in their genome, which are able to grow on agar plates and can be coaxed to differentiate into transgenic tobacco plants. un 10 25 imi
Transcribed Image Text:The generation of a transgenic plant Engineered T-DNA Tobacco-plant cell T-DNA inserts into a plant chromosome Transformed cell kan cells selected Transgenic Cultured cells Cell of transgenic plant tobacco Plantlet plant FIGURE 10-25 The insertion of T-DNA into plant chromosomes. Incubation of leaf disks with the bacterium A. tumefaciens plasmid. When the bacterium infects a plant cell, a part of the Ti plasmid is trans- ferred and inserted, apparently more or less at random, into the genome of the host plant (Figure 10-24). The region of the Ti plasmid that inserts into the host plant is called T-DNA, for transfer DNA. The genes whose products catalyze this T-DNA transfer reside in a region of the Ti plasmid separate from the T-DNA region itself. The natural behavior of the Ti plasmid makes it well suited to the role of a vector for plant genetic engineering. In particular, any DNA that is inserted between the T-DNA border (24-bp ends) sequences can be mobilized by other functions pro- vided by the Ti plasmid and inserted into plant chromosomes. Thus, scientists were able to eliminate all of the T-DNA sequence between the borders (including the tumor-causing genes) and replace it with the gene(s) of interest and a selectable marker (for example, kanamycin resistance). One method of introducing the T-DNA containing an engineered T-DNA leads to leaf cells with the T-DNA in their genome, which are able to grow on agar plates and can be coaxed to differentiate into transgenic tobacco plants. un 10 25 imi
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