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- After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.What is the best descriptive match for the following culture media? A. Specific biological characteristics allow this media to select specific groups of bacteria. B. All the components are well known. C. Will contain components that can inhibit certain groups of bacteria. D. Can be used to grow difficult growing or nutritional complex bacteria. E. This media contains amino acids for growing bacteria. select 1. Synthetic media select - 2. Selective media select - 3. Enriched media select 4. Differential media select - 5. Complex mediaUsing a batch culture method, the effects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension cultures
- A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential mediumc. defined mediumd. enriched medium1. Phenylethyl alcohol (PEA) agar is a selective media. Which of the following organisms should NOT grow well on PEA agar? Choose all that apply. Klebsiella pneumoniae Staphylococcus aureus Escherichia coli Streptococcus pneumoniae Bacillus cereus 2. Phenol red, a pH indicator, is a component of some differential media. Phenol red is a red at neutral and basic pHs and turns yellow at acidic pHs. Which of the following conditions would result in a yellow color in a medium containing lactose and phenol red? A.Growth of an organism that ferments glucose to alcohol. B. Growth of an organism that ferments mannitol but not lactose. C. Growth of an organism that ferments lactose to lactic acid. D. Growth of an organism that is not capable of fermenting any sugars.In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
- Outline a method for preparation of tomato cell suspension culture and subcultureThere are media that can be both differential and selective. what are 3 examples and the function of each one of them?In not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.
- distinguish between selective, differential, enriched, and minimal media. Give examples of Distinguish between selective, differential, enriched, and minimal. Give examples of each. know on what the selective and differential properties are based.For each of the following situations, describe how bacteria on these growth. media would appear on the plate: A. On MacConkey agar, a bacterium that grows on the medium but is negative for lactose fermentation B. On EMB agar, a bacterium that grows on the medium but is positive for lactose fermentation C. On mannitol salt agar, a bacterium that failed to grow on the medium D. On mannitol salt agar, a bacterium that grew on the medium and fermented mannitolExplain why micropropagation of banana is a better way of producing planting materials than traditional methodologies. Describe how to carry out micropropagation for this particular crop, including the type of explant to be used and the use of phytohormones or additives in the culture medium needed for each culture stage, along with justifications.