Homework Help is Here – Start Your Trial Now!
Engineering
Chemical Engineering
The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50

The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
See similar textbooks
icon
Related questions
Question
The following data were obtained for an enzyme-catalyzed reaction. Determine \( V_{\text{max}} \) and \( K_m \) by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows: \[ \begin{array}{|c|c|} \hline [S] \, \text{mol/L} & v \, \mu\text{mol/min} \\ \hline 5.0 \times 10^{-4} & 125 \\ 2.0 \times 10^{-4} & 125 \\ 6.0 \times 10^{-5} & 121 \\ 4.0 \times 10^{-5} & 111 \\ 3.0 \times 10^{-5} & 96.5 \\ 2.0 \times 10^{-5} & 62.5 \\ 1.6 \times 10^{-5} & 42.7 \\ 1.0 \times 10^{-5} & 13.9 \\ 8.0 \times 10^{-6} & 7.50 \\ \hline \end{array} \]
Transcribed Image Text:The following data were obtained for an enzyme-catalyzed reaction. Determine \( V_{\text{max}} \) and \( K_m \) by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows: \[ \begin{array}{|c|c|} \hline [S] \, \text{mol/L} & v \, \mu\text{mol/min} \\ \hline 5.0 \times 10^{-4} & 125 \\ 2.0 \times 10^{-4} & 125 \\ 6.0 \times 10^{-5} & 121 \\ 4.0 \times 10^{-5} & 111 \\ 3.0 \times 10^{-5} & 96.5 \\ 2.0 \times 10^{-5} & 62.5 \\ 1.6 \times 10^{-5} & 42.7 \\ 1.0 \times 10^{-5} & 13.9 \\ 8.0 \times 10^{-6} & 7.50 \\ \hline \end{array} \]
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 3 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The
SEE MORE TEXTBOOKS