The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50
The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows [S] mol/L 5.0 x 10-4 2.0 x 10-4 6.0 x 10-5 4.0 x 10-5 3.0 x 10-5 2.0 x 10-5 1.6 x 10-5 1.0 x 10-5 8.0 x 10-6 v μmol/min 125 125 121 111 96.5 62.5 42.7 13.9 7.50
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![The following data were obtained for an enzyme-catalyzed reaction. Determine \( V_{\text{max}} \) and \( K_m \) by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows:
\[
\begin{array}{|c|c|}
\hline
[S] \, \text{mol/L} & v \, \mu\text{mol/min} \\
\hline
5.0 \times 10^{-4} & 125 \\
2.0 \times 10^{-4} & 125 \\
6.0 \times 10^{-5} & 121 \\
4.0 \times 10^{-5} & 111 \\
3.0 \times 10^{-5} & 96.5 \\
2.0 \times 10^{-5} & 62.5 \\
1.6 \times 10^{-5} & 42.7 \\
1.0 \times 10^{-5} & 13.9 \\
8.0 \times 10^{-6} & 7.50 \\
\hline
\end{array}
\]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe80ef817-aa8b-4047-ac27-a3f534c77dd1%2Fa48cc4ee-4ec4-4bce-9c4d-c07f76f9c85b%2Fw1muqfo_processed.png&w=3840&q=75)
Transcribed Image Text:The following data were obtained for an enzyme-catalyzed reaction. Determine \( V_{\text{max}} \) and \( K_m \) by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows:
\[
\begin{array}{|c|c|}
\hline
[S] \, \text{mol/L} & v \, \mu\text{mol/min} \\
\hline
5.0 \times 10^{-4} & 125 \\
2.0 \times 10^{-4} & 125 \\
6.0 \times 10^{-5} & 121 \\
4.0 \times 10^{-5} & 111 \\
3.0 \times 10^{-5} & 96.5 \\
2.0 \times 10^{-5} & 62.5 \\
1.6 \times 10^{-5} & 42.7 \\
1.0 \times 10^{-5} & 13.9 \\
8.0 \times 10^{-6} & 7.50 \\
\hline
\end{array}
\]
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