Propose an appropriate intermediate for the following reaction The figure below shows an absorption spectrum of DNA obtained usingNanoDrop. Is this DNA preparation?à 230 240 250 260 270 280 290 300 310 320 330 340 350a. Contaminated with proteins.b. Contaminated with RNA?c. This is a band spectrum of pure DNA (no protein contamination).d.This is not a band absorption spectrum of DNA.

Answered: The figure below shows an absorption…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Propose an appropriate intermediate for the following reaction

The figure below shows an absorption spectrum of DNA obtained using
NanoDrop. Is this DNA preparation?
à 230 240 250 260 270 280 290 300 310 320 330 340 350
a. Contaminated with proteins.
b. Contaminated with RNA?
c. This is a band spectrum of pure DNA (no protein contamination).
d.
This is not a band absorption spectrum of DNA.

Expert Solution

Step 1

DNA and RNA are the nucleic acids that are made up of nucleotides. The concentration of these biomolecules in biological samples can be determined by one of the most important techniques called nano drop technique, which is a spectrophotometric analysis. The technique can measure the concentration and purity of nucleic acids at a very low concentration level ( Ex: less than 10 ng/ microlitre)