The enzyme aspartate transcarbamoylase (ATCase) catalyzes the reaction below and, as discussed in class and in the text, is a prototypic allosteric enzyme. س سال سے بیاد ملی NH3 Asp + H₂N OPO3 CP ATCase H₂N ΝΗ + HPO4 a. Classify this enzyme into one of the 4 categories of biochemical reactions. b. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the native enzyme and enzyme that has been treated with a mercurial. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5. Briefly explain why these plots are different. c. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the enzyme in the absence of effectors and in the presence of CTP and ATP. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5- d. Sketch, on a single plot, separate curves of velocity vs [CTP] and velocity vs [ATP]. In both cases assume low concentrations (e. < K0.5) o both aspartate and carbamoyl phosphate.
The enzyme aspartate transcarbamoylase (ATCase) catalyzes the reaction below and, as discussed in class and in the text, is a prototypic allosteric enzyme. س سال سے بیاد ملی NH3 Asp + H₂N OPO3 CP ATCase H₂N ΝΗ + HPO4 a. Classify this enzyme into one of the 4 categories of biochemical reactions. b. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the native enzyme and enzyme that has been treated with a mercurial. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5. Briefly explain why these plots are different. c. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the enzyme in the absence of effectors and in the presence of CTP and ATP. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5- d. Sketch, on a single plot, separate curves of velocity vs [CTP] and velocity vs [ATP]. In both cases assume low concentrations (e. < K0.5) o both aspartate and carbamoyl phosphate.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please answer all parts in detail. Give explainations. Thanks.
![The enzyme aspartate transcarbamoylase (ATCase) catalyzes the reaction below and, as discussed in class and in the text, is a prototypic
allosteric enzyme.
+
NH3
Asp
+
на доров
H₂N
CP
ATCase
lup
ΝΗ
H₂N
+
20
HPO4
a. Classify this enzyme into one of the 4 categories of biochemical reactions.
b. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the native enzyme and enzyme that has been treated with a
mercurial. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5. Briefly explain why these plots are
different.
c. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the enzyme in the absence of effectors and in the presence of
CTP and ATP. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5.
d. Sketch, on a single plot, separate curves of velocity vs [CTP] and velocity vs [ATP]. In both cases assume low concentrations (e. < K0.5) of
both aspartate and carbamoyl phosphate.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ffeae0ed4-2929-4bbe-a3af-ffb225aa6bed%2F01a123ac-e82f-469b-bea6-d4cd64dc5331%2Fs0sicbn_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The enzyme aspartate transcarbamoylase (ATCase) catalyzes the reaction below and, as discussed in class and in the text, is a prototypic
allosteric enzyme.
+
NH3
Asp
+
на доров
H₂N
CP
ATCase
lup
ΝΗ
H₂N
+
20
HPO4
a. Classify this enzyme into one of the 4 categories of biochemical reactions.
b. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the native enzyme and enzyme that has been treated with a
mercurial. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5. Briefly explain why these plots are
different.
c. Sketch, on a single plot, separate curves of the velocity vs. [aspartate] for the enzyme in the absence of effectors and in the presence of
CTP and ATP. You should assume that [carbamoyl phosphate] is the same for each curve, and is < Ko.5.
d. Sketch, on a single plot, separate curves of velocity vs [CTP] and velocity vs [ATP]. In both cases assume low concentrations (e. < K0.5) of
both aspartate and carbamoyl phosphate.
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Step 1: The 4 main categories of biochemical reactions
VIEWStep 2: (a) ATCase catalyze a condensation reaction
VIEWStep 3: (b) Velocity v/s [aspartate] plot for native and mercurial treated ATCase
VIEWStep 4: (c) Velocity v/s [aspartate] plot for ATCase in the absence and presence of effectors (ATP & CTP)
VIEWStep 5: (d) Velocity v/s [ATP] and velocity v/s [CTP] curves for ATCase
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