The compound known as nitrous acid is a reactive chemical that replaces amino groups (NH2) with keto groups (=O).  When nitrous acid reacts with the bases in DNA, it can change cytosine to uracil and change adenine to hypoxanthine.  A DNA double helix has the following sequence: AACCTACGACC TTGGATGCTGG   A.   What would be the sequence of this double helix immediately after reaction with the nitrous acid (i.e. before replication)?  Let the letter H represent hypoxanthine and U represent uracil.  B.   Following exposure the nitrous acid was removed, and the DNA was replicated for one generation. What would be the sequence of the DNA products after the DNA sequence resulting from treatment in Part A had replicated once?  Your answer should contain the sequences of two double stranded DNA molecules. Note:  During DNA replication, uracil hydrogen bonds with adenine, and hypoxanthine hydrogen bonds with cytosine.

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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The compound known as nitrous acid is a reactive chemical that replaces amino groups (NH2) with keto groups (=O).  When nitrous acid reacts with the bases in DNA, it can change cytosine to uracil and change adenine to hypoxanthine.  A DNA double helix has the following sequence:

AACCTACGACC

TTGGATGCTGG

 

A.  
What would be the sequence of this double helix immediately after reaction with the nitrous acid (i.e. before replication)?  Let the letter H represent hypoxanthine and U represent uracil. 

B.  
Following exposure the nitrous acid was removed, and the DNA was replicated for one generation. What would be the sequence of the DNA products after the DNA sequence resulting from treatment in Part A had replicated once?  Your answer should contain the sequences of two double stranded DNA molecules. Note:  During DNA replication, uracil hydrogen bonds with adenine, and hypoxanthine hydrogen bonds with cytosine.

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