The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS. Combine the following: • 80g NaCl • 2g KCl • 14.4g Na2HPO4 (dibasic anhydrous) • 2.4g KH2PO4 (monobasic anhydrous) • 800mL distilled H2O 1. Adjust pH to 7.4 with HCl 2. Add H2O to 1L 3. Autoclave for 20 minutes on liquid cycle. Store at room temperature. Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?
The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS. Combine the following: • 80g NaCl • 2g KCl • 14.4g Na2HPO4 (dibasic anhydrous) • 2.4g KH2PO4 (monobasic anhydrous) • 800mL distilled H2O 1. Adjust pH to 7.4 with HCl 2. Add H2O to 1L 3. Autoclave for 20 minutes on liquid cycle. Store at room temperature. Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS.
Combine the following:
• 80g NaCl
• 2g KCl
• 14.4g Na2HPO4 (dibasic anhydrous)
• 2.4g KH2PO4 (monobasic anhydrous)
• 800mL distilled H2O
1. Adjust pH to 7.4 with HCl
2. Add H2O to 1L
3. Autoclave for 20 minutes on liquid cycle. Store at room temperature.
Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?
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