The competition host, Chuck Charles, sampled each burger as part of the judging; later that evening, he came down with a severe case of what is determined to be food poisoning. Jimmy Pesto claims that Bob’s weird burger must have been the culprit, but Louise is determined to show this isn’t the case, and that Jimmy’s oregano burger was the cause of the illness. How can Louise scientifically demonstrate that the cause of Chuck’s illness was not from her dad’s garlic burger, but instead from Jimmy Pesto’s oregano burger? In other words, describe Louise’s experimental approach, and data/results, to rule in the oregano burger, AND rule out the garlic burger, as the cause of Chuck Charles’ illness.

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.