The chemical 5-amino-2,3-dihydro-1,4- phthalazinedione, better known as luminol, is used by forensic scientists in analyzing crime scenes for the presence of washed-away blood. Luminol is so sensitive that it can detect blood that has been diluted 10,000 times. A basic solution of luminol is often sprayed onto surfaces that are suspected of containing minute amounts of blood. Luminol has a molecular weight of 177 g/mol. The forensic technician at a crime scene has just prepared a luminol stock solution by adding 17.0 g of luminol into a total volume of 75.0 mL of H₂O. What is the molarity of the stock solution of luminol? Express your answer with the appropriate units. ▸ View Available Hint(s) molarity of luminol solution = 1.28 M Submit Previous Answers ✓ Correct Part B Before investigating the scene, the technician must dilute the luminol solution to a concentration of 4.00 x 10-2 M. The diluted solution is then placed in a spray bottle for application on the desired surfaces. How many moles of luminol are present in 2.00 L of the diluted spray? Express your answer with the appropriate units. ▸ View Available Hint(s) moles of luminol = 8.00x10-² mol Submit Previous Answers ✓ Correct Part C What volume of the stock solution (Part A) would contain the number of moles present in the diluted solution (Part B)? Express your answer with the appropriate units. ▸ View Available Hint(s) volume = ...Å Value 1 Units ?

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**Crime Scene Analysis Using Luminol**

**Understanding Luminol:**
The chemical 5-amino-2,3-dihydro-1,4-phthalazinedione, widely known as luminol, is crucial in forensic science for analyzing crime scenes by detecting washed-away blood. Luminol can identify blood traces that have been diluted up to 10,000 times. It is frequently sprayed onto surfaces suspected of containing minuscule amounts of blood. Luminol has a molecular weight of 177 g/mol.

**Luminol Stock Solution Preparation:**
- A forensic technician prepares a luminol stock solution by dissolving 17.0 g of luminol in a total volume of 75.0 mL of water (\(H_2O\)).
- **Calculation of Molarity:**  
  The molarity of the luminol stock solution is calculated to be 1.28 M. This calculation is verified as correct.

**Part B: Dilution for Scene Investigation:**
- Prior to investigating, the luminol solution must be diluted to a concentration of \(4.00 \times 10^{-2} \, M\).
- This diluted solution is applied using a spray bottle on surfaces of interest.
- **Moles in Diluted Spray:**  
  In a 2.00 L volume of this diluted solution, the moles of luminol present are \(8.00 \times 10^{-2}\) mol. This answer is correct as per the instructions.

**Part C: Volume of Stock Solution Required:**
- **Objective:** Determine the volume of stock solution from Part A that would contain the number of moles present in the diluted solution from Part B.
- The task is to express the volume with the appropriate units.

This exercise demonstrates the preparation and application of luminol in practical forensic work, highlighting the importance of accurate calculations in creating working solutions for crime scene investigations.
Transcribed Image Text:**Crime Scene Analysis Using Luminol** **Understanding Luminol:** The chemical 5-amino-2,3-dihydro-1,4-phthalazinedione, widely known as luminol, is crucial in forensic science for analyzing crime scenes by detecting washed-away blood. Luminol can identify blood traces that have been diluted up to 10,000 times. It is frequently sprayed onto surfaces suspected of containing minuscule amounts of blood. Luminol has a molecular weight of 177 g/mol. **Luminol Stock Solution Preparation:** - A forensic technician prepares a luminol stock solution by dissolving 17.0 g of luminol in a total volume of 75.0 mL of water (\(H_2O\)). - **Calculation of Molarity:** The molarity of the luminol stock solution is calculated to be 1.28 M. This calculation is verified as correct. **Part B: Dilution for Scene Investigation:** - Prior to investigating, the luminol solution must be diluted to a concentration of \(4.00 \times 10^{-2} \, M\). - This diluted solution is applied using a spray bottle on surfaces of interest. - **Moles in Diluted Spray:** In a 2.00 L volume of this diluted solution, the moles of luminol present are \(8.00 \times 10^{-2}\) mol. This answer is correct as per the instructions. **Part C: Volume of Stock Solution Required:** - **Objective:** Determine the volume of stock solution from Part A that would contain the number of moles present in the diluted solution from Part B. - The task is to express the volume with the appropriate units. This exercise demonstrates the preparation and application of luminol in practical forensic work, highlighting the importance of accurate calculations in creating working solutions for crime scene investigations.
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