The carboxylate may still decarboxylate in the environment of the enzyme active site. - The carboxylate maybe converted to the acid form in the environment of the enzyme active site pri o decarboxylation. The carboxylate may still decarboxylate in the environment of the enzyme active site due to the resence of NAD+.

Chemistry
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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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c. An enol is the nucleophile while an enolate is the electrophile.
d. A carbonyl group (aldehyde or ketone) is the electrophile while an enolate is the nucleophile.
23. The organic chemist decarboxylates beta-ketoacids at acidic pH values. In the mitochondria the pH is
around 7. What does this mean in terms of the mechanism of beta-ketoacid acid decarboxylation of
isocitrate in the enzyme active site?
a. The carboxylate may still decarboxylate in the environment of the enzyme active site.
b. The carboxylate maybe converted to the acid form in the environment of the enzyme active site prior
to decarboxylation.
c. The carboxylate may still decarboxylate in the environment of the enzyme active site due to the
presence of NAD+.
d. Both a & b maybe correct.
24. In the Krebs cycle isocitrate is oxidized to oxalosuccinic acid (also called alpha-ketoglutarate). What
else happens in this step?
a. NADH is oxidized to NAD+.
b. NAD+ is reduced to NADH.
c. Isocitrate decarboxylates.
d. both b & c
Transcribed Image Text:c. An enol is the nucleophile while an enolate is the electrophile. d. A carbonyl group (aldehyde or ketone) is the electrophile while an enolate is the nucleophile. 23. The organic chemist decarboxylates beta-ketoacids at acidic pH values. In the mitochondria the pH is around 7. What does this mean in terms of the mechanism of beta-ketoacid acid decarboxylation of isocitrate in the enzyme active site? a. The carboxylate may still decarboxylate in the environment of the enzyme active site. b. The carboxylate maybe converted to the acid form in the environment of the enzyme active site prior to decarboxylation. c. The carboxylate may still decarboxylate in the environment of the enzyme active site due to the presence of NAD+. d. Both a & b maybe correct. 24. In the Krebs cycle isocitrate is oxidized to oxalosuccinic acid (also called alpha-ketoglutarate). What else happens in this step? a. NADH is oxidized to NAD+. b. NAD+ is reduced to NADH. c. Isocitrate decarboxylates. d. both b & c
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