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The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca²+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.) Kg = 110

The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca²+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.) Kg = 110

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1x10-6 M.) %3D Kd 110
Transcribed Image Text:The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1x10-6 M.) %3D Kd 110
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