Table 2 below contains a set of data on batch fermentation of Saccharomyces cerevisiae (wild-strain) using 100g/L of glucose as an initial substrate concentration that acts as a carbon source. This fermentation process is used to produce amino acid. Table 2: Data of batph-culture of Saccharomyces cerevisiae for amino acid production 2 Amino Acid Concentration Time (h) Cell Concentration GI Concentration L-13 Nitrogen Concentration (all)
Table 2 below contains a set of data on batch fermentation of Saccharomyces cerevisiae (wild-strain) using 100g/L of glucose as an initial substrate concentration that acts as a carbon source. This fermentation process is used to produce amino acid. Table 2: Data of batph-culture of Saccharomyces cerevisiae for amino acid production 2 Amino Acid Concentration Time (h) Cell Concentration GI Concentration L-13 Nitrogen Concentration (all)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Transcribed Image Text:QUESTION 6
Table 2 below contains a set of data on batch fermentation of Saccharomyces cerevisiae
(wild-strain) using 100g/L of glucose as an initial substrate concentration that acts as a
carbon source. This fermentation process is used to produce amino acid.
Table 2: Data of batphculture of Saccharomyces cerevisiae for amino acid production
2
(+)
Amino Acid
Concentration
(g/L)
0
Time
(h)
0
1
2
3
4
J
S
6
7
8
Cell
Concentration
(g/L)
0.20
0.70
1.70
2.90
4.50
5.50
5.60
5.70
5.70
0
6
15
30
49
69
87
107
a) Analyze the specific growth rate (μ).
Glucose
Concentration
(g/L)
100
96
87
b) Calculate the maximum specific growth rate (μm).
c) Determine the growth yield coefficient.
d) Determine the kinetics of amino acid formation.
79
69
13
1.05
1
0
END OF QUESTION PAPER
Nitrogen
Concentration
(g/L)
10.0
9.20
7.40
5.50
3.00
1.20
0.50
0.10
0
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