Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter40: Nuclear Medicine And Molecular Imaging
Section: Chapter Questions
Problem 6RQ
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Transcribed Image Text:PRELAB: Calculating Magnification
Each lens makes the object look larger by a specified amount.
To
determine
the
total
magnification, multiply the
magnification of the eyepiece and the objective being used.
Table 1: Microscope Magnification
Magnifying power
Objective
Objective
Eyepiece
Total
name
Scanner
4 X
Low power
100 X
High power
40 χ
PRELAB: Estimating the Size of an Object using a
Microscope
You will not be able to use a ruler to directly measure the size
of a cell.
Instead, you will use an indirect method by
comparing the cell to the size of something you already know.
The diameter of the microscope's field of view as seen
through the eyepiece is a convenient length to use.
A very small unit of measurement, the micron (µm) is used
for measuring microscopic objects. The conversions are:
1 um = 0.001 mm
1 mm = 1000 µm
Table 2: Field of View Diameters
Objective
Diameter
name
millimeter (mm)
micron (um)
Scanner
4
Low power
High power
0.5
To estimate the size of an object, divide the diameter by the
approximate number of specimens that could fit across
that diameter.
For example, if you estimate that 20 cells could fit across the
diameter when viewing under low power, then the size is:
diameter
amt of objects
2000 un
20 cells
100 Hn/cell
%3D
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