Suppose you need to fill an agarose gel tank with 400 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How many milliliters of the 50X stock solution should you combine with water to make 400 ml of 1X TAF?

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Suppose you need to fill an agarose gel tank with 400
mL of TAE buffer. Unfortunately, one of your labmates
was inconsiderate and used up all of the 1X TAE buffer
without making more. Fortunately, you have found a
bottle of 50X TAE stock solution. How many milliliters
of the 50X stock solution should you combine with
water to make 400 mL of 1X TAE?
mL
Transcribed Image Text:Suppose you need to fill an agarose gel tank with 400 mL of TAE buffer. Unfortunately, one of your labmates was inconsiderate and used up all of the 1X TAE buffer without making more. Fortunately, you have found a bottle of 50X TAE stock solution. How many milliliters of the 50X stock solution should you combine with water to make 400 mL of 1X TAE? mL
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Step 1

Here We use the volume strength formula ,

that is , V1S1=V2S2

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