Suppose that the thermal denaturation experiment on the protein with the Met to Lys mutation took place at pH 10.0. Would the mutation have a greater or lesser impact on stability at pH 10.0 compared to pH 3.0? no change, because the Lys side chain pK, is above 10.0 so the side chain remains charged lesser, because a less acidic environment contributes to general protein stability lesser, because a greater fraction of Lys can remain buried in the core at pH 10.0 greater, because high pH values destabilize proteins
Suppose that the thermal denaturation experiment on the protein with the Met to Lys mutation took place at pH 10.0. Would the mutation have a greater or lesser impact on stability at pH 10.0 compared to pH 3.0? no change, because the Lys side chain pK, is above 10.0 so the side chain remains charged lesser, because a less acidic environment contributes to general protein stability lesser, because a greater fraction of Lys can remain buried in the core at pH 10.0 greater, because high pH values destabilize proteins
Chapter12: Water Requirements For Aquaculture
Section: Chapter Questions
Problem 7KA
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Transcribed Image Text:The figure shows an example of a thermal denaturation plot.
The melting temperature (Tm) is the midpoint of the
temperature range over which denaturation occurs.
Percent unfolded
100
80
60
40
20
0
20
Tm
40
60
Temperature (°C)
80
100
Transcribed Image Text:Suppose that the thermal denaturation experiment on the protein with the Met to Lys mutation took place at pH 10.0.
Would the mutation have a greater or lesser impact on stability at pH 10.0 compared to pH 3.0?
no change, because the Lys side chain pK, is above 10.0 so the side chain remains charged
lesser, because a less acidic environment contributes to general protein stability
lesser, because a greater fraction of Lys can remain buried in the core at pH 10.0
greater, because high pH values destabilize proteins
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