Spinach chromatography lab: provide a short description of the following processes: a) Spinach treatment b) Preparing a chromatography column c) Collecting the two major specifies (β-carotene and Chlorophyll A) d) Spectra measurement

Q: If a single spot divides into 3 unique spots during a paper chromatography experiment,  how many Rf…

A: Here we are required to find number of Rf that can be calculated when a single spot divide into 3…

Q: 23. What would cause compounds with similar structures to be separates on a TLC plate as a…

A: The detail explaination to the above question is given in step 2.

Q: Which compound from each pair would be collected first when purifying a mixture using column…

A: We know that

Q: When spiking a sample with isopentyl alcohol, the isopentyl alcohol peak should increase in area on…

A: It is a calibration method that involves adding an additional amount of the substance of interest to…

Q: ) Is this more likely to occur at low analyte concentrations or high analyte concentrations? Explain…

A: Stray light (spectral purity) gives potentially rise to incorrect absorbance measurements.

Q: Which ion reaches the detector (cathode) first in capillary electrophoresis (CE)? a) Nat b) cr c)…

A: The speed of ions moving towards cathode in capillary electrophoresis depends of factors like net…

Q: 2. A mixture of acid compounds is analyzed by paper chromatography. Use the centimeter uler to…

A: Retention  factor is used for  sperating mixtures and identifying the Compounds.

Q: A student separates colored compounds A and B (shown in grey in the figure below) using column…

A: A question based on analytical process. Based on the Column chromatography and the polarity index,…

Q: A thermal conductivity detector (TCD) is used in a) HPLC b) GC c) size exclusion chromatography d)…

A: Thermal conductivity detector works by measuring changes in the thermal conductivity of the carrier…

Q: Which technique measures secondary structure? a. Size exclusion chromatography b. Circular Dichroism…

A: The secondary structure is formed by bending of the 1o structure. In this, an intermolecular force…

Q: Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate…

A: Apparatus diagram

Q: One of the following techniques cannot be used for separation ounodwom Select one: O a.…

A: Answer - The correct option is (b) boiling point determination. The boiling point of a compound,…

Q: In column chromatography, dyes with higher polarities tend to attach to which of the forbwing? A.…

A: The correct option is (B). Column chromatography= Column chromatography is a technic which is used…

Q: What type of chromatography is referred to as molecular (size) exclusion chromatography? a) HP.L.C.…

A: Size exclusion chromatography is the process which separate molecules on the basis of size.

Q: Please correct answer and don't use hend raiting

A: To calculate the amount of PBS needed to dilute the eluate from an affinity chromatography run, you…

Q: Regarding TLC: a) Compounds A and B are analyzed side-by-side by TLC on silica gel. The developed…

A: Thin layer chromatography is a type of adsorption chromatography for the separation of components…

Q: Explain the following terms. a) Calibration curves b) Chromophores

A: A calibration curve is used to assesshow well the classifier is calibrated. H. How different are the…

Q: Please don't provide handwritten solution ....

A: Thin layer chromatography (TLC) is a method of separating components of the mixture. It is based on…

Q: 5. Two food colors, A and B, have similar structure and similar polarities and a chromatogram for…

A: Chromatography is the analytical technique used to separate the mixture of compounds. The mixture or…

Q: 6) You are given a mixture of organic compounds suspected of containing various amines (e. g.…

A: Solvent extraction- It is a technique used to separate compounds based on their relative solubility…

Q: Why do we use more than one solvent in the cold extraction method?

A: In chemistry like dissolves like

Q: it about 1 cm from one end. Using colored marke spots of color on the center of the line. lass…

A: Concepts of Thin Layer Chromatography.

Q: ii) What are the adjusted retention times for components A and B? iv) What is the relative retention…

A: 3 . For component A = 8-1.2=6.8 For component B = 10-1.2 = 8.8 4. Separation factor = 1.29

Q: 2. Do compounds always elute in the same order on a TLC plate and on chromatography column? Why or…

A: TLC stands for thin layer chromatography

Q: Can you TYPE the definitions of the all of words please Chromatography TLC Stationary phase Mobile…

A: Most of the time, the compounds we encounter are not in their purest form. They are essentially a…

Q: Q11: Which ion chromatography conditions is suitable to isolate a protein that has a surface rich in…

A: Ion chromatography is a method that can be used to separate and purify proteins based on their…

Q: 3 Compare: a) Capillary Electrophoresis with GC and HPLC. b) Separation Versus Preconcentration.

A:

Q: 2. Regarding the extraction experiment, which of the following statements is true? a) A drying agent…

A: TLC, or Thin Layer Chromatography, is a chromatographic technique used to separate and analyze…

Q: In the Thermo Fisher application note about wine analysis (Lesson 3), the following chromatogram was…

A: Resolution factor  shows accuracy of quantitative analysis

Q: how might chromatography be used to analyze the composition of the dye in a marker?

A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: Question:  What will happen to the result of chromatography if both the stationary and mobile…

A: Chromatography  is the  technique  in which the mixture are separated using   mobile and stationary…

Q: Are there methods other than spectrophotometry to determine protein concentration? If there is,…

A: Protein Concentration: Protein concentration is referred to as the overall amount and content of a…

Q: 1. For the following chromatography state what the mobile phase is, stationary phase is, and what…

A: Chromatography is a versatile analytical technique used for the separation and identification of…

Q: What are your observations performing paper chromatography

A: Paper chromatography is of two types . Thin layer chromatography Preparative TLC

Q: Flavonols are a type of flavonoids, in a silica gel stationary phase chromatography, with what kind…

A: Silica gel is SiO2, which means it has polar covalent bonds and it will interact with the polar…

Q: 1- The melting point experiment is a good technique for.. a) Extraction b) Identification c)…

A: The temperature at which solid changes its state to liquid at atmospheric pressure is called the…

Q: Peak Retention Time (minutes) Area 1.201 136501204 acetone 2.011 1253056 toluene ortho-product…

A:

Q: Is the mobile phase for your TLC and column chromatography more or less polar than the stationary…

A: Mobile phase: It is the phase that is getting movement in the column. The sample that has to be…

Q: How might the Rf values of colorless substances be determined? That is, how could the colorless…

A: we use locating agents which reacts with the substance to form colored compound

Q: 4. If the protein reacts with the blue (580 nm) dye to form a purple color (550 nm) complex, draw…

A: Protein reacts with blue dye to form a purple complex. We have to draw diagram to show how…

Q: Item 29: Question What is the relative retention (a) for compounds A and B if the retention time for…

A: Given data:tA= 3.91 mintB= 6.42 mintm= 1.34 minFormula for relative retention,

Q: by

A: Chromatography is basically that technique which allows the separation, identification, and…

Q: calculate Rfs for each spot on your chromatogram

A: Retention factor is equal to the ratio of distance travelled by solute divided by the distance…

Q: Answer as established by thin ayer chromatography: 1. The value of Rf will always be: A. less…

A: To solve this problem we have to explain about Rf value  in chromatography