Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,
Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Question
100%
Transcribed Image Text:Solute
Blue dextran
Molecular Weight
of solute Log' of
the #
Na2CrO4
2,000,000
Alkaline
phosphatase
Cytochrome C| 12,400
86,000
162
Log (Molecular wt.)
6.3
4.93
4.09
2.20
Elution Volume Ve (ml)
0.5M) AI
65 -6
2:3ML
3.5Ml
1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue
dextran. Why?
V- 3.14 r²h
Vt =
Measure inner radius of the column, r =
Measure the Height of the column bed, h=
2. Calculate the total volume occupied by the column Vt using following equation and data
provided,
AI-Generated Solution
AI-generated content may present inaccurate or offensive content that does not represent bartleby’s views.
Unlock instant AI solutions
Tap the button
to generate a solution
Recommended textbooks for you
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY