Sketch energy level diagrams for a 3-level and a 4-level laser system indicating specifically the pumping, lasing, and fast decay transitions in each case. 04= 6. (10) Radiant power of the fluorescence is expressed during an experiment using the approximate expression derived in the class: DL 2.303kD.ebc. For a 10 M sample with &=103 M'cm in a 1-cm cuvette, what would be the % error in the Q value because of the use of this approximate expression? 9.667 O RO-E -1-30366

A photometer with a linear response to radiation gave a reading of 690 mV with a blank in the light path and 169 mV when the blank was replaced by an absorbing solution. Calculate *(a) the transmittance and absorbance of the absorbing solution. (b) the expected transmittance if the concentration of absorber is one half that of the original solution. *(c) the transmittance to be expected if the light path through the original solution is doubled.

The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.

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Given g(x, y, z)= e sin(-2x) cos(y), millimis memen VEM Ple Cre COMET The wor M Pa ex ma

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Indicate the relationship between 1 eV and 8065.54 cm-1.

Radiation of wavelength 280 nm passed through 1.0 mm of a solution that contained an aqueous solution of the amino acid tryptophan at a concentration of 0.50 mmol dm-3 . The intensity of the rad iation is reduced to 54 per cent of its init ial va lue (so T = 0.54). Calcu late the molar absorpt ion coefficient and the absorbance of tryptophan at 280 nm. What would be the transmittance through a cell of thickness 2.0 mm?

An analyst needs to evaluate the potential effect of an interferent, I, on the quantitative analysis for an analyte, A. She begins by measuring the signal for a sample in which the interferent is absent and the analyte is present with a concentration of 15 ppm, obtaining an average signal of 23.3 (arbitrary units). When analyzing a sample in which the analyte is absent and the interferent is present with a concentration of 25 ppm, she obtains an average signal of 13.7.

An analyst needs to evaluate the potential effect of an interferent, I, on the quantitative analysis for an analyte, A. She begins by measuring the signal for a sample in which the interferent is absent and the analyte is present with a concentration of 15 ppm, obtaining an average signal of 23.3 (arbitrary units). When analyzing a sample in which the analyte is absent and the interferent is present with a concentration of 25 ppm, she obtains an average signal of 13.7. What is the sensitivity for the analyte? What is the sensitivity for the interfering? What is the value of the selectivity coefficient? What is the maximum concentration of interferent relative to that of the analyte (i.e. interferent/analyte), if the error in the analysis is to be less than 1%?