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SHOW COMPLETE SOLUTIONS Q3. Which isolate has A260/280 ratio of above 2.0?

SHOW COMPLETE SOLUTIONS Q3. Which isolate has A260/280 ratio of above 2.0?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Q3. Which isolate has A260/280 ratio of above 2.0?

The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotometeric measurements. Absorbance reading at 320 provides a general measurement of the turbidity of the sample. The organic compounds (containing aromatic rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0 -2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (µg/mL) = (A260 reading - A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength. Analyze the data given below and determine the following: Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1.27 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32
Transcribed Image Text:The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotometeric measurements. Absorbance reading at 320 provides a general measurement of the turbidity of the sample. The organic compounds (containing aromatic rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0 -2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (µg/mL) = (A260 reading - A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength. Analyze the data given below and determine the following: Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1.27 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32
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