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Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution Final [NaCI] (mM) number 1 150 120 90 4 60 30 6.

Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution Final [NaCI] (mM) number 1 150 120 90 4 60 30 6.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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PART A Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Final [NaCl] (mM) Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution number 1 150 2 120 90 4 60 30 6
Transcribed Image Text:PART A Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Final [NaCl] (mM) Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution number 1 150 2 120 90 4 60 30 6
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