questions in pictures Sex the flies and note the colour of eyes in all F2 flies.Record the observations in the table4. ObservationsNo. of Flies (Parents for mating)No. ofMalesGenerationDate ofNo. ofMatingFemalesMalesFemalesRedWhileRedWhileEyeEyeEyeEyeParents101010000010Results after matingF1Date ofDate ofDate ofNo. of Flies (F1 generation)separating hatching hatchingforAdultfor1st F12nd F1MalesFemalesрarentfliesBatchBatchRedWhileRedWhileEyeEyeEyeEyeGenerationDate ofNo. ofNo. ofNo. of Flies (Parents for mating)MatingMalesFemalesMalesFemalesRedWhileRedWhileEyeEyeEyeEyeF1101000101000Results after selfing of F1 fliesDate ofF2Date ofDate ofNo. of Flies (F2 generation)18separating hatching hatchingforAdult F1forflies1st F22nd F2MalesFemalesBatchBatchRedWhileRedWhileEyeEyeEyeEye5. ResultsF1 GenerationRed eyed female White eyed femaleRed eyed maleWhite eyed maleNo. of FliesRatioF2 GenerationRed eyed female White eyed femaleRed eyed maleWhite eyed maleNo. of FliesRatio19 LAB 3. To demonstrate sex-linked inheritance of eye-colour in Drosophila melanogaster.1. IntroductionIf a trait is located on “X" chromosome, the trait would get expressed in alternate sex from onegeneration to the other. The inheritance pattern depends on which parent carries the gene on the Xchromosome. The trait or character can be transmitted from father to daughter because X-chromosomeof father is always received by daughter. The trait can be transmitted from mother to son or mother todaughter because X- chromosome of mother is distributed between both daughter and son. This is calledCris-Cross inheritance. The recessive trait normally gets expressed in male only since the female carriesa double dose of X chromosome, and therefore she remains a carrier.2. Requirements:Drosophila melanogaster cultures (including wild and eye colour mutants), Ether, Binocularmicroscope, Food culture bottle, fly etherizer, soft hairbrush, glassware etc.3. Procedure3.1. Collecting Virgin Female flies:Remove all flies from the culture vessel a day before the pupae become ready to hatch (Fliesnormally emerge on 11th or 12th day after eggs are laid).• Check the surface of food for any remaining flies.• Collect newly emerged adult virgin flies early in the morning, 7:00 am – 8:00 am (flies emergein early morning after sunrise and mate within 8 hours).Anesthetize the flies and drop them on a filter paper.• Identify the male flies (darker abdomen) and female flies (lighter abdomen) and isolate thefemale flies. (Virgin flies are with soft bodies and should be handled with soft hair brush)• Identify and separate virgin female flies as follows;a. Virgin female flies have much larger abdomens.b. Virgin females have a dot on the side of thec. Females with folded wings and wet must have just emerged and will be virgin.abdomen.173.2. Crossing mutant fliesCollect 10 white eyed (mutant) virgin females and 10 red eyed (wild type) males.• Keep them together in a fly culture bottle with food for 7 days.On eight day, remove all adult flies.• Check for eggs laid and keep the culture bottle for aside for hatching ofAs the flies emerge (12th day after eggs are laid) collect the flies early morning as virgin flies.This is F1 generation.Sex the flies and note the colour of eyes in all F1 flies.In F1 generation, all male flies will be white eyed while all female flies will be red eyed.Isolate ten males and ten female flies and transfer them into one fly culture bottle with food.Leave them undisturbed for seven days.On eight day, remove all adult F1 flies.Check for eggs laid and keep the culture bottle aside for hatching of eggs.As the flies emerge (12th day after eggs are laid), collect the flies early morning as virgin flies.This is F2 generation.eggs.18

Drosophila melanogaster is defined as a small, common fly that is found close to unripe and rotted…

Sex the flies and note the colour of eyes in all F2 flies. Record the observations in the table 4. Observations No. of Flies (Parents for mating) Generation| Date of Mating No. of Males No. of Females Males Females Red While Red While Eye 10 Eye 00 Eye 00 Eye 10 Parents 10 10 Results after mating F1 Date of Date of Date of No. of Flies (F1 generation) separating hatching hatching for 2nd F1 Adult for 1st F1 Batch Males Females parent flies Batch Red While Red While Eye Eye Eye Eye Generation Date of No. of Males No. of Females No. of Flies (Parents for mating) Mating Males While Females Red Red While Eye 00 Eye 10 Eye Eye 00 F1 10 10 10 Results after selfing of F1 flies Date of F2 Date of Date of No. of Flies (F2 generation) 18 separating Adult F1 for flies hatching hatching for 2nd F2 Batch 1st F2 Batch Males Females Red While Red While Eye Eye Eye Eye 5. Results F1 Generation Red eyed female White eyed female Red eyed male White eyed male No. of Flies Ratio F2 Generation Red eyed female White eyed female Red eyed male White eyed male No. of Flies Ratio

Sex the flies and note the colour of eyes in all F2 flies. Record the observations in the table 4. Observations No. of Flies (Parents for mating) Generation| Date of Mating No. of Males No. of Females Males Females Red While Red While Eye 10 Eye 00 Eye 00 Eye 10 Parents 10 10 Results after mating F1 Date of Date of Date of No. of Flies (F1 generation) separating hatching hatching for 2nd F1 Adult for 1st F1 Batch Males Females parent flies Batch Red While Red While Eye Eye Eye Eye Generation Date of No. of Males No. of Females No. of Flies (Parents for mating) Mating Males While Females Red Red While Eye 00 Eye 10 Eye Eye 00 F1 10 10 10 Results after selfing of F1 flies Date of F2 Date of Date of No. of Flies (F2 generation) 18 separating Adult F1 for flies hatching hatching for 2nd F2 Batch 1st F2 Batch Males Females Red While Red While Eye Eye Eye Eye 5. Results F1 Generation Red eyed female White eyed female Red eyed male White eyed male No. of Flies Ratio F2 Generation Red eyed female White eyed female Red eyed male White eyed male No. of Flies Ratio

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter10: Control Of Gene Expression

Section: Chapter Questions

Problem 3CT: Almost all calico cats (one is pictured in FIGURE 10.7B) are female. Why? B When this calico cat was...

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Almost all calico cats (one is pictured in FIGURE 10.7B) are female. Why? B When this calico cat was an embryo, one of the two X chromosomes was inactivated in each of her cells. The descendants of the cells formed her adult body, which is a mosaic for expression of X chromosome genes. Black fur arises in patches where genes on the X chromosome inherited from one parent are expressed; orange fur arises in patches where genes on the X chromosome inherited from the other parent are expressed. FIGURE 10.7 Animated X chromosome inactivation.
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