Scientist generate a Tris buffer using powders for both the weak acid (Tris-HCl) and conjugate base (Tris-Cl-). Now they have been tasked to make a 200 ml 0.15 M Tris buffer ( however, they found that in the chemical cupboard there is only Tris base (Tris-Cl-) powder but the container with weak acid powder is empty. The cupboard does, however, also contain bottles of strong bases and strong acids. i) explain how they can generate a functional buffer although they lack the Tris-HCl weak acid powder. Hint: explain what is necessary to obtain a functional buffer system? Here we do not ask for calculations but for a conceptual account on how the buffer can be made with only the Tris base as starting material. ii) Calculate in grams (g) to three decimals precision the mass of Tris base (Tris-Cl-) powder that is required to generate the desired buffer.
Scientist generate a Tris buffer using powders for both the weak acid (Tris-HCl) and conjugate base (Tris-Cl-). Now they have been tasked to make a 200 ml 0.15 M Tris buffer ( however, they found that in the chemical cupboard there is only Tris base (Tris-Cl-) powder but the container with weak acid powder is empty. The cupboard does, however, also contain bottles of strong bases and strong acids.
i) explain how they can generate a functional buffer although they lack the Tris-HCl weak acid powder. Hint: explain what is necessary to obtain a functional buffer system? Here we do not ask for calculations but for a conceptual account on how the buffer can be made with only the Tris base as starting material.
ii) Calculate in grams (g) to three decimals precision the mass of Tris base (Tris-Cl-) powder that is required to generate the desired buffer.
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