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ris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and K₁ is .19 x 10-6 for the aqueous reaction (HOCH2)3 CN H2 + H₂O (HOCH₂)3 CN H3+ + OH¯ TRIS TRISH+ a. What is the optimal pH for TRIS buffers? pH = b. Calculate the ratio [TRIS]/[TRISH+] at pH = 7.00 [TRIS] TRISH and at pH=9.00. [TRIS] [TRISH+] (Simplify your answer completely.) c. A buffer is prepared by diluting 50.0 g TRIS base and 35.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? pH = What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer? pH =

ris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and K₁ is .19 x 10-6 for the aqueous reaction (HOCH2)3 CN H2 + H₂O (HOCH₂)3 CN H3+ + OH¯ TRIS TRISH+ a. What is the optimal pH for TRIS buffers? pH = b. Calculate the ratio [TRIS]/[TRISH+] at pH = 7.00 [TRIS] TRISH and at pH=9.00. [TRIS] [TRISH+] (Simplify your answer completely.) c. A buffer is prepared by diluting 50.0 g TRIS base and 35.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? pH = What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer? pH =

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and K₁ is 1.19 x 10-6 for the aqueous reaction (HOCH2)3 CN H2 + H₂O ⇒ (HOCH2)3 CN H3 + + OH™ TRIS TRISH+ a. What is the optimal pH for TRIS buffers? pH b. Calculate the ratio [TRIS]/[TRISH+] at pH = 7.00 [TRIS] [TRISH+] = 9.00. [TRIS] TRISH+] (Simplify your answer completely.) c. A buffer is prepared by diluting 50.0 g TRIS base and 35.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? pH = What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer? pH = and at pH
Transcribed Image Text:Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and K₁ is 1.19 x 10-6 for the aqueous reaction (HOCH2)3 CN H2 + H₂O ⇒ (HOCH2)3 CN H3 + + OH™ TRIS TRISH+ a. What is the optimal pH for TRIS buffers? pH b. Calculate the ratio [TRIS]/[TRISH+] at pH = 7.00 [TRIS] [TRISH+] = 9.00. [TRIS] TRISH+] (Simplify your answer completely.) c. A buffer is prepared by diluting 50.0 g TRIS base and 35.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? pH = What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer? pH = and at pH
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