The organic compound trishydroxymethylaminomethane is abbreviated Tris and is basic. The conjugate acid (TrisH+) has a pKa of 8.10. TrisH++ H20 = Tris + H30+ Tris is commonly used as a component in buffer solutions to study biochemical reactions. Part A A buffer solution is prepared by mixing 12.5 mL of 0.100 mol L-'HCI with 25 mL of 0.100 mol L-'Tris. What the pH of this buffer? Express your answer using two decimal places. Templates Symbols uado redo reset keyboard shortcuts help pH = Submit Request Answer • Part B The buffer was used to study an enzyme-catalyzed reaction in which 0.00030 mol of H30+ was produced. What is the pH of the solution after the acid reacted with the buffer? Express your answer using two decimal places. Templates Symbols uado redo reset keyboard shortcuts help, pH =

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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The organic compound trishydroxymethylaminomethane is abbreviated Tris and is basic. The conjugate acid (TrisH+) has a pKa of 8.10.
TrisHt+ H20= Tris + H30+
Tris is commonly used as a component in buffer solutions to study biochemical reactions.
Part A
A buffer solution is prepared by mixing 12.5 mL of 0.100 mol L'HCl with 25 mL of 0.100 mol L-lTris. What is the pH of this buffer?
Express your answer using two decimal places.
femplates Symbols uado redo reset keyboard shortcuts help,
pH =
Submit
Request Answer
Part B
The buffer was used to study an enzyme-catalyzed reaction in which 0.00030 mol of H30+was produced. What is the pH of the solution after the acid reacted with the buffer?
Express your answer using two decimal places.
Templates Symbols uado redo reset keyboard shortcuts help
pH =
Transcribed Image Text:The organic compound trishydroxymethylaminomethane is abbreviated Tris and is basic. The conjugate acid (TrisH+) has a pKa of 8.10. TrisHt+ H20= Tris + H30+ Tris is commonly used as a component in buffer solutions to study biochemical reactions. Part A A buffer solution is prepared by mixing 12.5 mL of 0.100 mol L'HCl with 25 mL of 0.100 mol L-lTris. What is the pH of this buffer? Express your answer using two decimal places. femplates Symbols uado redo reset keyboard shortcuts help, pH = Submit Request Answer Part B The buffer was used to study an enzyme-catalyzed reaction in which 0.00030 mol of H30+was produced. What is the pH of the solution after the acid reacted with the buffer? Express your answer using two decimal places. Templates Symbols uado redo reset keyboard shortcuts help pH =
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