Recommend a buffering capacity and maximum buffering capacity for each buffer. Start by locating their corresponding pKa values in your textbooks or online sources. (eg. Campbell's Biochemistry, 9th ed or the latest ed.). Buffer Components Ammonium/Ammonia buffer Lactic acid/Lactate buffer Phosphoric acid/hydrogen PO4 buffer Benzoic acid/Benzoate buffer pk value Answer: Why? Buffering Capacity Maximum Buffering Capacity Follow-up question: Which of these buffers would you use if you need to carry out a PCR reaction using Taq polymerase which has an optimum pH at 7.2? Suggest only one buffer.

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Recommend a buffering capacity and maximum buffering capacity for each buffer. Start
by locating their corresponding pKa values in your textbooks or online sources. (eg.
Campbell's Biochemistry, 9th ed or the latest ed.).
Buffer Components
Ammonium/Ammonia buffer
Lactic acid/Lactate buffer
Phosphoric acid/hydrogen PO4 buffer
Benzoic acid/Benzoate buffer
pk value
Answer:
Why?
Buffering
Capacity
Maximum
Buffering
Capacity
Follow-up question:
Which of these buffers would you use if you need to carry out a PCR reaction using
Taq polymerase which has an optimum pH at 7.2? Suggest only one buffer.
Transcribed Image Text:Recommend a buffering capacity and maximum buffering capacity for each buffer. Start by locating their corresponding pKa values in your textbooks or online sources. (eg. Campbell's Biochemistry, 9th ed or the latest ed.). Buffer Components Ammonium/Ammonia buffer Lactic acid/Lactate buffer Phosphoric acid/hydrogen PO4 buffer Benzoic acid/Benzoate buffer pk value Answer: Why? Buffering Capacity Maximum Buffering Capacity Follow-up question: Which of these buffers would you use if you need to carry out a PCR reaction using Taq polymerase which has an optimum pH at 7.2? Suggest only one buffer.
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