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QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?

QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?
Transcribed Image Text:QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?
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