Question Completion Status: You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You contiguous sequence, you examine it for any other protein coding genes. You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel Image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. PUC-233 Chromosome 7q31.1 H XRAY H H --300kb- H- Hind!!! 600bp 900bp Colony Colony Colony Colony Colony Colony Insert Check-Gel of your samples Which colony(-les) is (are) likely to have the XRAY gene? O a. Colonies 1, 2, 3 & 4 O b. Colony 2 O c. Colony 1 O d. Colonies 5 & 6 Oe. Colonies 3 & 4 FOXP2 1500bp andard H

Biology 2e
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ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 37CTQ: An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding...
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Question Completion Status:
You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to bo linked to a variant of the FOXP2 gene. You have identified the
minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You
identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You
want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid
(pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing
the Xray gene (gel Image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question.
POD-123
Chromosome 7q31.1
H XRAY H
1-30161
H- Hind!!!
600bp
900bp
Colony Colony Colony Colony Colony
Insert Check-Gel of your samples
Which colony(-les) is (are) likely to have the XRAY gene?
O a. Colonies 1, 2, 3 & 4
O b. Colony 2
O c. Colony 1
O d. Colonies 5 & 6
Oe. Colonies 3 & 4
FOXP2
1500bp
H
Transcribed Image Text:Question Completion Status: You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to bo linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel Image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. POD-123 Chromosome 7q31.1 H XRAY H 1-30161 H- Hind!!! 600bp 900bp Colony Colony Colony Colony Colony Insert Check-Gel of your samples Which colony(-les) is (are) likely to have the XRAY gene? O a. Colonies 1, 2, 3 & 4 O b. Colony 2 O c. Colony 1 O d. Colonies 5 & 6 Oe. Colonies 3 & 4 FOXP2 1500bp H
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