QUESTION 6 If you performed the experiment outlined in question 6 with full-leng would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen addec expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

question 6,7 are related to question 5 diagram. 

Please answer 6&7 both please

dutea Uu HUie Tormation
QUESTION 6
If you performed the experiment outlined in question 6 with full-length N-WASP + Actin + Arp2/3 what actin polymerization kinetics
would you observe (choose one of the curves in the graph above)?
Actin alone
Actin+Arp2/3+WHAMM
Actin+Arp2/3+WHAMM+2.0xRab1
QUESTION 7
"If you perform the experiment outlined in question 6 and tlen added CDC42 to the reaction, what polymerization curve do you
expect to see?"
Actin alone
Actin+Arp2/3+WHAMM
Actin+Arp2/3+WHAMM+2.0xRab1
Transcribed Image Text:dutea Uu HUie Tormation QUESTION 6 If you performed the experiment outlined in question 6 with full-length N-WASP + Actin + Arp2/3 what actin polymerization kinetics would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen added CDC42 to the reaction, what polymerization curve do you expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1
QUESTION 5
WHAMM is a nucleation promoting factor. Depicted above is a pyrene actin polymerization assay performed with the following
reaction conditions 1: Actin + Arp2/3 + WHAMM (active isoform); 2-5: Actin + Arp2/3 + WHAMM (active isoform) and increasing
concentrations of Rab1; or 6: actin alone. What effect does Rab1 have on branched actin polymerization?
Actin + Arp2/3 +
1.0
500nM WHAMM
+ 0.2x Rab1(M)
+ 1.0x Rab1(M)
+ 2.0x Rab1(M)
+ 3.0x Rab1(M)
Actin alone
0.5
0.0
1000
2000
Time (s)
Enhances WHAMM activity
inhibits WHAMM activity
No effect
induces actin bundle formation
F-actin (RFU)
Transcribed Image Text:QUESTION 5 WHAMM is a nucleation promoting factor. Depicted above is a pyrene actin polymerization assay performed with the following reaction conditions 1: Actin + Arp2/3 + WHAMM (active isoform); 2-5: Actin + Arp2/3 + WHAMM (active isoform) and increasing concentrations of Rab1; or 6: actin alone. What effect does Rab1 have on branched actin polymerization? Actin + Arp2/3 + 1.0 500nM WHAMM + 0.2x Rab1(M) + 1.0x Rab1(M) + 2.0x Rab1(M) + 3.0x Rab1(M) Actin alone 0.5 0.0 1000 2000 Time (s) Enhances WHAMM activity inhibits WHAMM activity No effect induces actin bundle formation F-actin (RFU)
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Bacterial infections
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON